EPF2 Antibody

Datasheet

Code	CSB-PA818527XA01DOA
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Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) EPF2 Polyclonal antibody

Uniprot No.

Q8LC53

Target Names

EPF2

Alternative Names

EPF2 antibody; At1g34245 antibody; F23M19Protein EPIDERMAL PATTERNING FACTOR 2 [Cleaved into: MEPF2] antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana EPF2 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

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Target Background

Function

Controls stomatal patterning. Regulates the number of cells that enter, and remain in, the stomatal lineage by inhibiting protodermal cells from adopting the meristemoid mother cell (MMC) fate in a non-cell-autonomous manner. Mediates stomatal development inhibition. MEPF2: mobile signal controlling stomatal development in a non-cell-autonomous manner. Uses ERECTA as major receptor. Inactivated by cleavage by CRSP (AC Q9LNU1). May act by competing with somatogen (AC Q9SV72) for the same receptor, TMM (AC Q9SSD1).

Gene References into Functions

In a ROS1 mutant, the promoter region of the peptide ligand gene EPF2 is hypermethylated, which reduces EPF2 expression and leads to overproduction of stomatal lineage cells. PMID: 24898766
the secreted protease CRSP, which cleaves the pro-peptide EPF2, in turn repressing stomatal development PMID: 25043023
Calculation of the number of stomata per leaf and leaf size showed that plants lacking EPF2 and EPFL9 had a higher proportion of non-stomatal epidermal cells. PMID: 22232766
Results suggest that EPF2 is expressed earlier in leaf development than EPF1 and is involved in determining the number of cells that enter, and remain in, the stomatal lineage. [EPF2] PMID: 19398336
EPF2 is a small secretory peptide expressed in meristemoid mother cells and regulates epidermal cell density. [EPF2] PMID: 19435754

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Subcellular Location

Secreted.

Protein Families

Plant cysteine rich small secretory peptide family, Epidermal patterning factor subfamily

Tissue Specificity

Expressed in leaves, especially by the MMCs and their early descendants cells (stomatal lineage cells) including guard mother cells (GMCs).

Database Links

KEGG: ath:AT1G34245

STRING: 3702.AT1G34245.1

UniGene: At.39780

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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EPF2 Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background