epmA Antibody

Code CSB-PA364196XA01ENV
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Product Details

Full Product Name
Rabbit anti-Escherichia coli (strain K12) epmA Polyclonal antibody
Uniprot No.
Target Names
epmA
Alternative Names
epmA antibody; genX antibody; poxA antibody; yjeA antibody; b4155 antibody; JW4116Elongation factor P--(R)-beta-lysine ligase antibody; EF-P--(R)-beta-lysine ligase antibody; EC 6.3.1.- antibody; EF-P post-translational modification enzyme A antibody; EF-P-lysine lysyltransferase antibody; GX antibody
Raised in
Rabbit
Species Reactivity
Escherichia coli (strain K12)
Immunogen
Recombinant Escherichia coli (strain K12) epmA protein
Immunogen Species
Escherichia coli (strain K12)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Protein A/G
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
With EpmB is involved in the beta-lysylation step of the post-translational modification of translation elongation factor P (EF-P) on 'Lys-34'. Catalyzes the ATP-dependent activation of (R)-beta-lysine produced by EpmB, forming a lysyl-adenylate, from which the beta-lysyl moiety is then transferred to the epsilon-amino group of EF-P 'Lys-34'. The substrate (R)-beta-lysine is 100-fold more efficient than either (S)-beta-lysine or L-alpha-lysine. Cannot ligate lysine to any tRNA.
Gene References into Functions
  1. Misacylation of yeast amber suppressor tRNA(Tyr) is via E. coli lysyl-tRNA synthetase. PMID: 16672269
Protein Families
Class-II aminoacyl-tRNA synthetase family, EpmA subfamily
Database Links
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Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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