Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) ERF1B Polyclonal antibody

Uniprot No.

Q8LDC8

Target Names

ERF1B

Alternative Names

ERF1B antibody; ERF092 antibody; ERF1 antibody; At3g23240 antibody; K14B15.15Ethylene-responsive transcription factor 1B antibody; AtERF1B antibody; Ethylene-responsive element-binding factor 1B antibody; EREBP-1B antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana ERF1B protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Acts as a transcriptional activator. Binds to the GCC-box pathogenesis-related promoter element. Involved in the regulation of gene expression during the plant development, and/or mediated by stress factors and by components of stress signal transduction pathways. Seems to be a key integrator of ethylene and jasmonate signals in the regulation of ethylene/jasmonate-dependent defenses. Can mediate resistance to necrotizing fungi (Botrytis cinerea and Plectosphaerella cucumerina) and to soil borne fungi (Fusarium oxysporum conglutinans and Fusiarium oxysporum lycopersici), but probably not to necrotizing bacteria (Pseudomonas syringae tomato).

Gene References into Functions

UBC18 negatively regulated drought and salt stress responses by altering the abundance of ERF1 and the expression of genes downstream of ERF1. PMID: 27787902
Using loss- and gain-of-function transgenic lines as well as biochemical analysis, we demonstrate that ERF1 can directly up-regulate ASA1 by binding to its promoter, leading to auxin accumulation and ethylene-induced inhibition of root growth. PMID: 26745809
Results provide evidence that AtERF1 is important for regulating AtNudt7 during oxidative signaling. PMID: 25451743
Expression of several ethylene biosynthetic genes and ethylene-responsive factors, including ERF1 and ERF2, was induced during reoxygenation. PMID: 24506560
ERF1 plays a positive role in salt, drought, and heat stress tolerance by stress-specific gene regulation, which integrates jasmonic acid, ethylene, and abscisic acid signals. PMID: 23719892
High- and medium-affinity binding sites are over-represented in promoters of MYC2- or ERF1-regulated genes, and therefore they may represent new cis-regulatory elements. PMID: 21284757

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Subcellular Location

Nucleus.

Protein Families

AP2/ERF transcription factor family, ERF subfamily

Tissue Specificity

Ubiquitously expressed, mostly in flowers and rosettes after ethylene treatment.

Database Links

KEGG: ath:AT3G23240

STRING: 3702.AT3G23240.1

UniGene: At.6341

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ERF1B Antibody

Product Details

Customer Reviews and Q&A

Target Background