SQE1 Antibody

Code CSB-PA287929XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) SQE1 Polyclonal antibody
Uniprot No.
Target Names
SQE1
Alternative Names
SQE1 antibody; DRY2 antibody; XF1 antibody; At1g58440 antibody; F9K23.3Squalene epoxidase 1 antibody; AtSQE1 antibody; EC 1.14.14.17 antibody; Protein DROUGHT HYPERSENSITIVE 2 antibody; Squalene monooxygenase antibody; XF1 protein antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana SQE1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Catalyzes the stereospecific oxidation of squalene to (S)-2,3-epoxysqualene, and is considered to be a rate-limiting enzyme in steroid biosynthesis. Can produce not only oxidosqualene, but also 2,3:22,23-dioxidosqualene. Main squalene epoxidase in the root. Sqe1 mutants may show defects in membrane lipid rafts, impairing the correct localization of RHD2 NADPH oxidase and the proper polarized production of ROS.
Gene References into Functions
  1. analysis of endomembrane dynamics showed that vesicle formation was affected in dry2/sqe1-5. Further analysis of proteins associated with sterol rich micro domains showed that dry2/sqe1-5 presented micro domains function altered. PMID: 28910707
  2. SQE1 encodes a squalene epoxidase protein involved in sterol biosynthesis, drought tolerance, and regulation of reactive oxygen species. PMID: 19309460
Subcellular Location
Membrane; Multi-pass membrane protein.
Protein Families
Squalene monooxygenase family
Tissue Specificity
Expressed in seedlings, leaves, stems, inflorescences, sepals, style and siliques. Expressed in expanded cotyledons, root tips and cortical cells of the root elongation zone, but not in root hair cells. In leaves, expressed in most cells, with a very stro
Database Links

KEGG: ath:AT1G58440

STRING: 3702.AT1G58440.1

UniGene: At.17240

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