Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) AZI1 Polyclonal antibody

Uniprot No.

Q9SU35

Target Names

AZI1

Alternative Names

AZI1 antibody; At4g12470 antibody; T1P17.60pEARLI1-like lipid transfer protein 1 antibody; Protein AZELAIC ACID INDUCED 1 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana AZI1 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Probable lipid transfer protein (LTP). Seems to control the flowering process and lignin synthesis. Together with DIR1, required for glycerol-3-phosphate- (G3P) and azelaic acid- (AA) induced systemic acquired resistance (SAR). Component of plant systemic immunity involved in priming defenses in a AA-dependent manner, by modulating production and/or translocation of a mobile signal(s) during SAR. Confers resistance to Botrytis cinerea and Pseudomonas syringae pv. tomato DC3000 and PmaDG3. May be involved in induced systemic resistance (ISR) mediated by non-pathogenic bacteria (e.g. P. fluorescens GM30). Prevents electrolyte leakage during freezing damage.

Gene References into Functions

used natural variation in Arabidopsis thaliana to study the role of zinc in regulating growth. We identify allelic variation of the systemic immunity gene AZI1 as a key for determining root growth responses to low zinc conditions PMID: 29608565
The function of AZI1 in systemic acquired resistance of Arabidopsis was confirmed by investigation of the phenotypic features of wild-type Col-0, AZI1 T-DNA knockout and AZI1 overexpressing plants after infection with virulent and avirulent Pseudomonas syringae. PMID: 27337039
AZI1 is a partially secreted protein and a likely new member of the group of hydroxyproline-rich glycoproteins. PMID: 26771603
plastid targeting of AZI1 and AZI1 paralogs occurs through a mechanism that may enable/facilitate their roles in signal mobilization PMID: 26203923
AZI1 is involved in defense priming in systemic plant immunity and was down-regulated in leaves by biotic and abiotic stresses. PMID: 23800991
The lipid transfer proteins AZI1 which is required for G3P- and azelaic acid-induced Systemic acquired resistance, is essential for G3P accumulation. PMID: 23602565
AZI1 might be multifunctional and associated with cold tolerance of Arabidopsis. PMID: 21492954
findings show that azelaic acid and AZI1 (At4g12470) are components of plant systemic immunity involved in priming defenses PMID: 19342588

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Subcellular Location

Secreted, cell wall. Endoplasmic reticulum. Cell junction, plasmodesma. Plastid, chloroplast.

Protein Families

Plant LTP family, PEARLI1 subfamily

Database Links

KEGG: ath:AT4G12470

STRING: 3702.AT4G12470.1

UniGene: At.3227

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AZI1 Antibody

Product Details

Customer Reviews and Q&A

Target Background