ERO1 Antibody

Code CSB-PA244629XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) ERO1 Polyclonal antibody
Uniprot No.
Target Names
ERO1
Alternative Names
ERO1 antibody; YML130C antibody; YM4987.05CEndoplasmic oxidoreductin-1 antibody; EC 1.8.4.- antibody; Endoplasmic reticulum oxidoreductase protein 1 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) ERO1 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Essential oxidoreductase that oxidizes proteins in the endoplasmic reticulum to produce disulfide bonds. Acts by oxidizing directly PDI1 isomerase through a direct disulfide exchange. Does not act as a direct oxidant of folding substrate, but relies on PDI1 to transfer oxidizing equivalent. Also able to oxidize directly the PDI related protein MPD2. Does not oxidize all PDI related proteins, suggesting that it can discriminate between PDI1 and related proteins. Reoxidation of ERO1 probably involves electron transfer to molecular oxygen via FAD. Acts independently of glutathione. May be responsible for a significant proportion of reactive oxygen species (ROS) in the cell, thereby being a source of oxidative stress.
Gene References into Functions
  1. Balanced Ero1 activation and inactivation establishes ER redox homeostasis. PMID: 22412017
  2. the intramolecular electron transfer from the a domain to the a' domain within PDI during its oxidation by ERO1alpha. PMID: 21757736
  3. Data of the crystal structure revealed local changes compared to the wild-type enzyme around the sites of mutation, but no conformational transitions within 25 A of the active site were observed. PMID: 20669236
  4. PDI has a role in native disulfide bond formation in a reaction driven by Ero1p PMID: 16368681
  5. These findings provide insight into mechanisms for regenerating oxidized Ero1p and maintaining disulfide bond formation under anaerobic conditions in the endoplasmic reticulum PMID: 16407158
  6. Ero1p mutants that have the surprising ability to oxidize substrates in the absence of Cys100-Cys105 are described. PMID: 16495342
  7. Study found that hyperoxidation of the endoplasmic reticulum is prevented by attenuation of Ero1p activity through noncatalytic cysteine pairs. PMID: 17448992

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Subcellular Location
Endoplasmic reticulum membrane; Peripheral membrane protein; Lumenal side.
Protein Families
EROs family
Database Links

KEGG: sce:YML130C

STRING: 4932.YML130C

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