E(spl)m8-HLH Antibody

Code CSB-PA320181XA01DLU
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Product Details

Full Product Name
Rabbit anti-Drosophila melanogaster (Fruit fly) E(spl)m8-HLH Polyclonal antibody
Uniprot No.
Target Names
E(spl)m8-HLH
Alternative Names
E(spl)m8-HLH antibody; E(spl) antibody; m8 antibody; CG8365Enhancer of split m8 protein antibody; E(spl)m8 antibody
Raised in
Rabbit
Species Reactivity
Drosophila melanogaster (Fruit fly)
Immunogen
Recombinant Drosophila melanogaster (Fruit fly) E(spl)m8-HLH protein
Immunogen Species
Drosophila melanogaster (Fruit fly)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Participates in the control of cell fate choice by uncommitted neuroectodermal cells in the embryo. Transcriptional repressor. Binds DNA on N-box motifs: 5'-CACNAG-3'. Part of the Notch signaling pathway.
Gene References into Functions
  1. Studies identify PP2A-Wdb as a participant in Notch signaling, and suggest that M8 activity is controlled by phosphorylation and dephosphorylation. PMID: 25006677
  2. Based on these data, we propose a model in which Hes genes are responsible for co-ordinating the Notch response of a wide spectrum of other targets, explaining the critical functions these key regulators play in many developmental and disease contexts. PMID: 23300480
  3. The phosphorylation domain of M8 is implicated in autoinhibition, and indicate that binding of the co-repressor Groucho (Gro) is context-dependent. PMID: 21789514
  4. Drosophila STUbL protein Degringolade limits HES functions during embryogenesis. PMID: 21486924
  5. Studies provide evidence that autoinhibition by the CtD regulates M8 activity in a phosphorylation-dependent manner. PMID: 20014344
  6. Polymorphisms in the enhancer of split gene complex affect bristle number variation in a large wild-caught cohort of Drosophila melanogaster. PMID: 16143618

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Subcellular Location
Nucleus.
Database Links

KEGG: dme:Dmel_CG8365

STRING: 7227.FBpp0084335

UniGene: Dm.27416

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