ESP1 Antibody

Code CSB-PA777713XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) ESP1 Polyclonal antibody
Uniprot No.
Target Names
ESP1
Alternative Names
ESP1 antibody; YGR098CSeparin antibody; EC 3.4.22.49 antibody; Separase antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) ESP1 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Caspase-like protease, which plays a central role in the chromosome segregation by cleaving the MCD1/SCC1 subunit of the cohesin complex at the onset of anaphase. During most of the cell cycle, it is inactivated by securin/PDS1 protein. It also promotes anaphase spindle elongation. A component of the FEAR (CDC14 early anaphase release) network which promotes CDC14 release from the nucleolus during early anaphase. Cleaves SLK19.
Gene References into Functions
  1. Esp1 is phosphorylated by Cdk1 and dephosphorylated by Cdc55.Esp1 role in the regulation pericentric cohesin and anaphase onset. PMID: 29561844
  2. We propose that Esp1 serves two roles to mediate Ty1 transposition - one to remove cohesin and the second to target Ty1-IN to chromatin PMID: 25822502
  3. phosphorylation either of separase or cohesin may be necessary for Rec8 cleavage PMID: 20230747
  4. Esp1 localizes to the mitotic spindle during anaphase. PMID: 18430955
  5. Esp1 is not essential for mitotic exit and contributes to the timing of mitotic exit mainly through promoting cohesin cleavage. PMID: 19144818
  6. Sgo1 can inhibit separase activity independently of securin. PMID: 19299562

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Subcellular Location
Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, spindle pole body. Cytoplasm. Note=Accumulates in the nucleus in G2 and is mobilized onto the spindle pole bodies and spindle midzone at anaphase onset, where it persists into midanaphase. The association with MCD1 may be important for its nuclear targeting.
Database Links

KEGG: sce:YGR098C

STRING: 4932.YGR098C

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