espP Antibody

Code CSB-PA765862XA01EOD
Size Enquire
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Full Product Name
Rabbit anti-Escherichia coli O157:H7 espP Polyclonal antibody
Uniprot No.
Target Names
espP
Alternative Names
espP antibody; L7020 antibody; ECO57PM78Serine protease EspP antibody; EC 3.4.21.-) [Cleaved into: Secreted autotransporter protein EspP antibody; Extracellular serine protease plasmid-encoded EspP); Autotransporter protein EspP translocator] antibody
Raised in
Rabbit
Species Reactivity
Escherichia coli O157:H7
Immunogen
Recombinant Escherichia coli O157:H7 espP protein
Immunogen Species
Escherichia coli O157:H7
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function
Serine protease capable of cleaving pepsin A and human coagulation factor V, which may contribute to the mucosal hemorrhage observed in hemorrhagic colitis.
Gene References into Functions
  1. Serine protease EspP from enterohemorrhagic Escherichia coli is sufficient to induce shiga toxin macropinocytosis in intestinal epithelium. PMID: 23874912
  2. Point mutations introduced at specific locations near the middle or N terminus of the EspP beta-helix that perturb folding also impair secretion, but to a lesser degree. PMID: 24165126
  3. Data show the completion of EspP beta domain assembly is regulated by an intrinsic checkpoint mechanism that requires the completion of passenger domain secretion. PMID: 21646511
  4. Efficient secretion of the passenger domain of the Escherichia coli O157:H7 autotransporter EspP requires the stable folding of a C-terminal approximately 17-kDa passenger domain segment. PMID: 20876094
  5. Adherence of E. coli O157:H7 was significantly impaired by espP mutation but restored upon addition of highly purified exogenous EspP. PMID: 17451446
  6. The data suggest that the EspP beta-domain and an embedded polypeptide segment are integrated into the outer membrane as a single pre-formed unit. PMID: 18047580

Show More

Hide All

Subcellular Location
[Serine protease EspP]: Periplasm.; [Secreted autotransporter protein EspP]: Secreted. Cell surface.; [Autotransporter protein EspP translocator]: Cell outer membrane; Multi-pass membrane protein.
Database Links

KEGG: ece:Z_L7020

STRING: 155864.L7020

icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2024 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1