ETO1 Antibody

Code CSB-PA528960XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) ETO1 Polyclonal antibody
Uniprot No.
Target Names
ETO1
Alternative Names
ETO1 antibody; At3g51770 antibody; ATEM1.2Ethylene-overproduction protein 1 antibody; Protein ETHYLENE OVERPRODUCER 1 antibody; Protein ETO1 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana ETO1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
Essential regulator of the ethylene pathway, which acts by regulating the stability of 1-aminocyclopropane-1-carboxylate synthase (ACS) enzymes. May act as a substrate-specific adapter that connects ACS enzymes, such as ACS5, to ubiquitin ligase complexes, leading to proteasomal degradation of ACS enzymes.
Gene References into Functions
  1. The mutant exhibits enhanced ethylene production via loss of function of ETHYLENE OVERPRODUCER1 (ETO1; whose normal function is to reduce ethylene production by degrading ethylene biosynthetic enzymes). PMID: 24064767
  2. Lack of ETO1 function confers soil-salinity tolerance through improved shoot Na/K homeostasis, effected via the ethylene resistant1-constitutive triple response1 ethylene signaling pathway. PMID: 24064768
  3. Treatment of etiolated Arabidopsis seedlings with light rapidly increases the stability of ACS5 protein and destabilizes the ETO1/EOLs proteins. PMID: 24065059
  4. The level of ETHYLENE-OVERPRODUCER1 (ETO1)/ETO1-LIKE (EOLs) in vivo plays a role in mediating ACS (1-aminocyclopropane-1-carboxylate synthase) protein turnover, with increased levels leading to a decrease in ACS protein levels. PMID: 23512855
  5. In Arabidopsis, ETO1 encodes a negative regulator of ethylene biosynthesis, and mutation of ETO1 causes Arabidopsis seedlings to overproduce ethylene. PMID: 22623414
  6. Ethylene overproduction partially restores auxin responsiveness to auxin-resistant mutants of ETO1. PMID: 21105933
  7. ETO1 (ETHYLENE-OVERPRODUCER1) is a negative regulator of type 2 1-aminocyclopropane-1-carboxylate synthases(ACC synthase). [ETO1] PMID: 16091151
  8. ETO1, EOL1 and EOL2 work together to negatively regulate ethylene synthesis, by helping degrade type-2 ACC synthases. PMID: 18808454
  9. In contrast to the wild type and ein3-1 mutants, both etr1-1 and eto1-1 mutants showed a sharp (up to 5-fold) increase in alpha-tocopherol levels during leaf aging. PMID: 19258016

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Protein Families
ETO1 family
Tissue Specificity
Predominantly expressed in flowers.
Database Links

KEGG: ath:AT3G51770

STRING: 3702.AT3G51770.2

UniGene: At.35356

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