ETR1 Antibody

Code CSB-PA344583XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) ETR1 Polyclonal antibody
Uniprot No.
Target Names
ETR1
Alternative Names
ETR1; At1g66340; T27F4.9; Ethylene receptor 1; AtETR1; Protein ETHYLENE RESPONSE 1; Protein ETR1
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana ETR1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Ethylene receptor related to bacterial two-component regulators. Acts as a redundant negative regulator of ethylene signaling. In the presence of ethylene, the auto-kinase activity of ETR1 is inhibited and the non-phosphorylated kinase domain binds tightly to the corresponding domain of EIN2.
Gene References into Functions
  1. ETR1-receiver domain already exists as an active formation and executes its function through binding with the downstream factors without phosphorylation. PMID: 27486797
  2. The ETR1 receiver domain has multiple functions where different surfaces are involved in the control of different traits. PMID: 26160962
  3. Data show that the cytosolic domain of ETR1 plays a key role, interacting with all other receptors and several participants of the ethylene signaling pathway. PMID: 25451923
  4. Data show that Bacillus sp B55, which associated with Nicotiana attenuata roots, promotes growth of wild-type and, particularly, ethylene (ET)-insensitive (35)S-ethylene response1 (etr1) plants expressing the mutant Arabidopsis thaliana receptor ETR1-1. PMID: 23903320
  5. Histidine kinase activity of ETR1 is not required for but plays a modulating role in the regulation of ethylene responses. PMID: 22467798
  6. Transgenic and genetic analyses revealed that this organelle abnormality was not linked to etr1-1 or ethylene signaling, but linked to a second mutation in ARC3, which was further verified by genetic complementation analysis. PMID: 22228186
  7. A mutation in the kinase domain of ETR1 that prevents auto-phosphorylation (H353A), increases the affinity of the receptors for EIN2. PMID: 20591837
  8. Impaired perception of ethylene via ETR1 results in increased disease resistance to Verticillium dahliae. PMID: 20447269
  9. Induction of etr1-1 expression repressed the epinastic response to ethylene as well as delayed fruit ripening. Restoration of ethylene sensitivity was achieved following the cessation of the induction PMID: 20181754
  10. Inhibition of autophosphorylation in ETR1 by cyanide in Arabidopsis thaliana can be controlled by etehylene or ethylene agonists. PMID: 19825547
  11. histidine kinase activity is not necessary for ethylene receptor function in plants and its signal transduction does not occur through a phosphorelay mechanism PMID: 15358768
  12. The His kinase domain in the C-terminal region of Arabidopsis ETR1 plays a role in the repression of ethylene responses. PMID: 15466228
  13. Five receptor isoforms from Arabidopsis thaliana confirmed that all members of the family are capable of high-affinity ethylene-binding activity. PMID: 15703053
  14. Data demonstrate an unexpected role for ETR1, that of mediating stomatal closure in response to H2O2. PMID: 15761208
  15. These findings demonstrate that RTE1 is a negative regulator of ethylene signaling and suggest that RTE1 plays an important role in ETR1 function. PMID: 16682642
  16. Possible roles of subfamily I receptors and disulfide linkages in ETR1 receptor signal output mediated through the N terminus are discussed. PMID: 16891553
  17. The ETR1 receptor is required for ethylene-stimulated nutations. PMID: 17071649
  18. These findings yield insight into the structure and function of the ethylene binding domain (EBD) and suggest a conserved role of the EBD as a negative regulator of the signal transmitter domain. PMID: 17189345
  19. The role of the histidine kinase activity of ETR1 in A. thaliana growth and development is reported. PMID: 17284582
  20. ETR1 is positively regulated by RTE1 via the ethylene-binding domain, independently from RAN1 activity. PMID: 18643990

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Subcellular Location
Endoplasmic reticulum membrane; Multi-pass membrane protein.
Protein Families
Ethylene receptor family
Tissue Specificity
Leaves, roots, stems, seedlings, flowers, anthers, carpels and ovules.
Database Links

KEGG: ath:AT1G66340

STRING: 3702.AT1G66340.1

UniGene: At.43238

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