eutL Antibody

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Code	CSB-PA301208XA01ENV
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Product Details

Full Product Name

Rabbit anti-Escherichia coli (strain K12) eutL Polyclonal antibody

Uniprot No.

P76541

Target Names

eutL

Alternative Names

eutL antibody; yffJ antibody; b2439 antibody; JW2432Ethanolamine utilization protein EutL antibody

Raised in

Rabbit

Species Reactivity

Escherichia coli (strain K12)

Immunogen

Recombinant Escherichia coli (strain K12) eutL protein

Immunogen Species

Escherichia coli (strain K12)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

A component of the bacterial microcompartment (BMC) shell dedicated to ethanolamine degradation (Probable). Two crystal forms have been seen; a form with a closed central pore that has 3 very small (1.1-2.2 Angstroms) channels per trimer lined by acidic and aromatic residues. A form with a large central pore (8-12 Angstroms) has also been seen; this is probably a functional pore which allows molecules to enter and exit the BMC in a selective, gated manner. Another group only sees the central pore in the presence of Zn(2+); soaking crystals in ZnCl(2) leads to dramatic conformational changes that open a central pore of about 12 Angstroms. Whether Zn(2+) binding is physiologically relevant is unclear, however it suggests a gating mechanism exists. Ethanolamine-binding by the small channels has been hypothesized to stabilize the EutL central pore in a closed (non-transporting) state. An open pore is thought to be large enough to transport ATP and/or cobalamin.

Gene References into Functions

In contrast to EutL, the pore of EutM appears to be positively charged, indicating specificity for different solutes. PMID: 20851901
the crystal structure of the shell protein EutL at 2.2-A resolution is presented. PMID: 19451619

Subcellular Location

Bacterial microcompartment.

Protein Families

EutL/PduB family

Database Links

KEGG: ecj:JW2432

STRING: 316385.ECDH10B_2604

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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eutL Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background