D15 Antibody

Code CSB-PA356750XA01FQT
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Product Details

Full Product Name
Rabbit anti-Escherichia phage T5 (Enterobacteria phage T5) D15 Polyclonal antibody
Uniprot No.
Target Names
D15
Alternative Names
D15 antibody; exo5 antibody; T5.130 antibody; T5p128Flap endonuclease antibody; FEN antibody; EC 3.1.11.- antibody; 5'-3' exonuclease antibody; Exodeoxyribonuclease antibody; EC 3.1.11.3 antibody
Raised in
Rabbit
Species Reactivity
Escherichia phage T5 (Enterobacteria phage T5)
Immunogen
Recombinant Escherichia phage T5 (Enterobacteria phage T5) D15 protein
Immunogen Species
Escherichia phage T5 (Enterobacteria phage T5)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Catalyzes both the 5'-exonucleolytic and structure-specific endonucleolytic hydrolysis of DNA branched nucleic acid molecules and probably plays a role in viral genome replication. Active on flap (branched duplex DNA containing a free single-stranded 5'-end), 5'overhangs and pseudo-Y structures. The substrates require a free, single-stranded 5' end, with endonucleolytic hydrolysis occurring at the junction of double- and single-stranded DNA. This function may be used for example to trim such branched molecules generated by Okazaki fragments synthesis during replication.
Gene References into Functions
  1. Direct observation of DNA threading in flap endonuclease complexes with DNA has been reported. PMID: 27273516
Database Links

KEGG: vg:2777611

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