Product Details

Full Product Name

Rabbit anti-Drosophila melanogaster (Fruit fly) E(z) Polyclonal antibody

Uniprot No.

P42124

Target Names

E(z)

Alternative Names

E(z) antibody; KMT6 antibody; CG6502Histone-lysine N-methyltransferase E(z antibody; EC 2.1.1.356 antibody; Lysine N-methyltransferase 6 antibody; Protein enhancer of zeste antibody

Raised in

Rabbit

Species Reactivity

Drosophila melanogaster (Fruit fly)

Immunogen

Recombinant Drosophila melanogaster (Fruit fly) E(z) protein

Immunogen Species

Drosophila melanogaster (Fruit fly)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Polycomb group (PcG) protein. Catalytic subunit of the Esc/E(z) complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. While PcG proteins are generally required to maintain the transcriptionally repressive state of homeotic genes throughout development, this protein is specifically required during the first 6 hours of embryogenesis to establish the repressed state. The Esc/E(z) complex is necessary but not sufficient for the repression of homeotic target genes, suggesting that the recruitment of the distinct PRC1 complex is also required.

Gene References into Functions

Taken together, these data suggest that E(z) acts intrinsically in germ cells to activate dedifferentiation and thus replenish lost germline stem cells during both aging and tissue regeneration. PMID: 28434938
Chracterization of functionally critical SU(Z)12 elements, including key determinants of SU(Z)12-E(Z) communication. PMID: 24100017
These results suggest that increased H3K27 trimethylation activity of E(Z)(Trm) causes the premature accumulation of H3K27me3 in early embryogenesis, "predestining" initially active Polycomb target genes to silencing once Polycomb silencing is initiated. PMID: 22182520
The emerging picture presents PRC2 as a cooperative multipart machine, intricately outfitted to sense and respond to the local chromatin environment and other cues[review] PMID: 22349693
Drosophila enhancer of Zeste/ESC complexes have a histone H3 methyltransferase activity that marks chromosomal Polycomb sites PMID: 12408863
The nucleosome-binding subunits Su(z)12 and Nurf55 anchor the E(z) enzyme on chromatin substrates, an essential process in maintaining HOX gene silencing during development. PMID: 15776017
one of the roles of ESC (and ESCL and EED) in Polycomb repressive complex 2 complexes is to enable E(Z) to utilize histone H3 as a substrate by physically linking enzyme and substrate PMID: 17210640

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Subcellular Location

Nucleus.

Protein Families

Class V-like SAM-binding methyltransferase superfamily, Histone-lysine methyltransferase family, EZ subfamily

Database Links

KEGG: dme:Dmel_CG6502

STRING: 7227.FBpp0306192

UniGene: Dm.2823

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E(z) Antibody

Product Details

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Target Background