F6'H1 Antibody

Datasheet

Code	CSB-PA124301XA01DOA
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Product Details
Customer Reviews and Q&A
Target Background

Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) F6'H1 Polyclonal antibody

Uniprot No.

Q9LHN8

Target Names

F6'H1

Alternative Names

F6'H1 antibody; At3g13610 antibody; K20M4.5Feruloyl CoA ortho-hydroxylase 1 antibody; EC 1.14.11.61 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana F6'H1 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Customer Reviews and Q&A

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Target Background

Function

2-oxoglutarate (OG)- and Fe(II)-dependent dioxygenase (2OGD) involved in scopoletin biosynthesis. Converts feruloyl CoA into 6'-hydroxyferuloyl CoA but has no activity with ferulic acid, feruloylquinic acid, caffeic acid, caffeoyl CoA, p-coumaric acid, cinnamic acid, cinnamoyl CoA or benzoyl CoA. Required for the production and secretion of compounds (e.g. fluorescent coumarins) that facilitate the mobilization and uptake of iron from sources with low bioavailability or in high pH-induced iron deficiency conditions. Involved in the pathway of sideretin biosynthesis from feruloyl CoA, a redox-active catecholic metabolite exuded by roots in response to iron deficiency in order to facilitate the uptake of iron; this pathway consists in the successive conversion from feruloyl CoA to scopoletin, from scopoletin to fraxetin and from fraxetin to sideretin. Catalyzes the biosynthesis of scopoletin from feruloyl CoA.

Gene References into Functions

Activity of F6'H1 towards 4-coumaroyl-CoA was not improved by introducing those mutations, indicating that besides TYR 151 and VAL 238, the subtle difference between the overall structures of F6'H1 and C2'H is also important for substrate specificity. PMID: 25993561
At3g13610 is essential for tolerance of Arabidopsis to high pH-induced Fe deficiency. PMID: 24246380
F6'H1/At3G13610 is a 2-oxoglutarate-dependent dioxygenase which synthesizes scopoletin by ortho-hydroxylation of feruloyl CoA. PMID: 18547395

Protein Families

Iron/ascorbate-dependent oxidoreductase family

Tissue Specificity

Highly expressed in roots, especially in the cortex.

Database Links

KEGG: ath:AT3G13610

STRING: 3702.AT3G13610.1

UniGene: At.48654

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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