F6'H1 Antibody

Code CSB-PA124301XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) F6'H1 Polyclonal antibody
Uniprot No.
Target Names
Alternative Names
F6'H1 antibody; At3g13610 antibody; K20M4.5Feruloyl CoA ortho-hydroxylase 1 antibody; EC antibody
Raised in
Species Reactivity
Arabidopsis thaliana
Recombinant Arabidopsis thaliana F6'H1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Purification Method
Antigen Affinity Purified
It differs from different batches. Please contact us to confirm it.
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Tested Applications
ELISA, WB (ensure identification of antigen)
Troubleshooting and FAQs
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

2-oxoglutarate (OG)- and Fe(II)-dependent dioxygenase (2OGD) involved in scopoletin biosynthesis. Converts feruloyl CoA into 6'-hydroxyferuloyl CoA but has no activity with ferulic acid, feruloylquinic acid, caffeic acid, caffeoyl CoA, p-coumaric acid, cinnamic acid, cinnamoyl CoA or benzoyl CoA. Required for the production and secretion of compounds (e.g. fluorescent coumarins) that facilitate the mobilization and uptake of iron from sources with low bioavailability or in high pH-induced iron deficiency conditions. Involved in the pathway of sideretin biosynthesis from feruloyl CoA, a redox-active catecholic metabolite exuded by roots in response to iron deficiency in order to facilitate the uptake of iron; this pathway consists in the successive conversion from feruloyl CoA to scopoletin, from scopoletin to fraxetin and from fraxetin to sideretin. Catalyzes the biosynthesis of scopoletin from feruloyl CoA.
Gene References into Functions
  1. Activity of F6'H1 towards 4-coumaroyl-CoA was not improved by introducing those mutations, indicating that besides TYR 151 and VAL 238, the subtle difference between the overall structures of F6'H1 and C2'H is also important for substrate specificity. PMID: 25993561
  2. At3g13610 is essential for tolerance of Arabidopsis to high pH-induced Fe deficiency. PMID: 24246380
  3. F6'H1/At3G13610 is a 2-oxoglutarate-dependent dioxygenase which synthesizes scopoletin by ortho-hydroxylation of feruloyl CoA. PMID: 18547395
Protein Families
Iron/ascorbate-dependent oxidoreductase family
Tissue Specificity
Highly expressed in roots, especially in the cortex.
Database Links

KEGG: ath:AT3G13610

STRING: 3702.AT3G13610.1

UniGene: At.48654

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