FAAH Antibody

Code CSB-PA007938XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) FAAH Polyclonal antibody
Uniprot No.
Target Names
FAAH
Alternative Names
FAAH; At5g64440; T12B11.3; Fatty acid amide hydrolase; AtFAAH; N-acylethanolamine amidohydrolase
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana FAAH protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Catalyzes the hydrolysis of bioactive endogenous fatty acid amides to their corresponding acids. The hydrolysis of endogenous amidated lipids terminates their participation as lipid mediators in various signaling systems (Probable). Converts a wide range of N-acylethanolamines (NAEs) to their corresponding free fatty acids and ethanolamine. Can use oleamide as substrate, but not indole-3-acetamide, 1-naphtalene-acetamide, nicotinic acid amide or L-asparagine. Can use 2-arachidonylglycerol as substrate. Participates in the regulation of plant growth. Hydrolyzes N-dodecanoylethanolamine, which is has a growth inhibitory effect on seedling growth. Involved in plant defense signaling. Involved in abscisic acid (ABA) signaling through mechanisms that are independent of the catalytic activity. Involved in the regulation of flowering time. Catalyzes the hydrolysis of N-acyl L-homoserine lactones (AHLs), which are a class of signaling molecules produced by bacteria for quorum sensing. Accumulation of L-homoserine appears to encourage plant growth at low concentrations by stimulating transpiration, but higher concentrations inhibit growth by stimulating ethylene production.
Gene References into Functions
  1. our study demonstrates the feasibility of using an unbiased chemical genetic approach to identify new pharmacological tools for manipulating FAAH- and NAE-mediated physiological processes in plants. PMID: 28112243
  2. AtFAAH is one, but not the only, modulator of endogenous N-Acylethanolamines (NAE) levels in plants, and that NAE depletion likely participates in the regulation of plant growth [fatty acid amide hydrolase] [AtFAAH] PMID: 16880402
  3. Overexpression of AtFAAH inhibits innate immunity against Pseudomonas syringae in Arabidopsis, and plants had lower amounts of jasmonic acid, abscisic acid, and salicylic acid. PMID: 18643971
  4. AtFAAH influences plant growth and interacts with ABA signaling and plant defense through distinctly different mechanisms PMID: 19801664
Subcellular Location
Endoplasmic reticulum membrane. Cell membrane.
Protein Families
Amidase family
Tissue Specificity
Expressed in roots, leaves and flowers. Expressed in seedlings, flowers, roots, siliques, seeds and leaves.
Database Links

KEGG: ath:AT5G64440

STRING: 3702.AT5G64440.1

UniGene: At.19619

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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