FAD3 Antibody

Code CSB-PA342958XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) FAD3 Polyclonal antibody
Uniprot No.
Target Names
FAD3
Alternative Names
FAD3; At2g29980; F23F1.10; Acyl-lipid omega-3 desaturase; cytochrome b5, endoplasmic reticulum; Omega-3 fatty acid desaturase 3, endoplasmic reticulum
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana FAD3 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Microsomal (ER) omega-3 fatty acid desaturase introduces the third double bond in the biosynthesis of 18:3 fatty acids, important constituents of plant membranes. It is thought to use cytochrome b5 as an electron donor and to act on fatty acids esterified to phosphatidylcholine and, possibly, other phospholipids.
Gene References into Functions
  1. Histone acetyltransferase general control non-repressed protein 5 (GCN5) affects the fatty acid composition of Arabidopsis thaliana seeds by acetylating fatty acid desaturase3 (FAD3). PMID: 27500884
  2. ALA10 interacts with FATTY ACID DESATURASE2 (FAD2), and modification of ALA10 expression affects phosphatidylcholine (PC) fatty acyl desaturation by disturbing the balance between FAD2 and FAD3 activities. PMID: 26620528
  3. SAD6 functions as a Delta9-desaturase, and together with FAD3 it increases the levels of unsaturated fatty acids in crown galls under hypoxia and drought stress conditions. PMID: 24368335
  4. FAD2 and FAD3 desaturases form heterodimers that facilitate metabolic channel. PMID: 24811169
  5. that bZIP67 binds G-boxes in the FAD3 promoter and enhances FAD3 expression. PMID: 23995083
  6. Increase in the dosage of FAD3 results in an increase in the linolenic acid content of seed oil. PMID: 21848868
  7. The fatty acid composition of membrane lipids influences cell growth and mitochondrial respiration, in particular the expression and capacity of alternative oxidase, under cold stress in fad2 knockout and FDAD3 overexpressing cell lines PMID: 17507388
  8. The polymethylene-interrupted dienoic fatty acid, mangiferic acid, can be produced in seed oil through the overexpression of a fatty acid n-3 desaturase. PMID: 19548018

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Subcellular Location
Endoplasmic reticulum membrane; Multi-pass membrane protein.
Protein Families
Fatty acid desaturase type 1 family
Tissue Specificity
Abundant in leaves and seedlings. Barely detectable in root tissue.
Database Links

KEGG: ath:AT2G29980

STRING: 3702.AT2G29980.1

UniGene: At.271

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