fadR Antibody

Code CSB-PA364231XA01ENV
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Product Details

Full Product Name
Rabbit anti-Escherichia coli (strain K12) fadR Polyclonal antibody
Uniprot No.
Target Names
fadR
Alternative Names
fadR antibody; oleR antibody; thdB antibody; b1187 antibody; JW1176Fatty acid metabolism regulator protein antibody
Raised in
Rabbit
Species Reactivity
Escherichia coli (strain K12)
Immunogen
Recombinant Escherichia coli (strain K12) fadR protein
Immunogen Species
Escherichia coli (strain K12)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Multifunctional regulator of fatty acid metabolism. Represses transcription of at least eight genes required for fatty acid transport and beta-oxidation including fadA, fadB, fadD, fadL and fadE. Activates transcription of at least three genes required for unsaturated fatty acid biosynthesis: fabA, fabB and iclR, the gene encoding the transcriptional regulator of the aceBAK operon encoding the glyoxylate shunt enzymes.
Gene References into Functions
  1. Elimination of the guanidine side chain of the CoA moiety binding site severely diminished the ability of FadR to bind the acyl-CoA effector. PMID: 26385696
  2. Structural analyses of FadR protein indicated that the hydrophobic interaction amongst the three amino acids (W60, F74 and W75) is critical for its DNA-binding ability. PMID: 25311842
  3. FadR activates the transcription of Fatty Acid synthesis genes. PMID: 25802297
  4. Regulates FadH together with ArcA-ARcB systen and the cyclic AMP receptor protein-cyclic AMP. PMID: 20622065
Subcellular Location
Cytoplasm.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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