fbf-2 Antibody

Code CSB-PA289833XA01CXY
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Product Details

Full Product Name
Rabbit anti-Caenorhabditis elegans fbf-2 Polyclonal antibody
Uniprot No.
Target Names
fbf-2
Alternative Names
fbf-2 antibody; F21H12.5Fem-3 mRNA-binding factor 2 antibody
Raised in
Rabbit
Species Reactivity
Caenorhabditis elegans
Immunogen
Recombinant Caenorhabditis elegans fbf-2 protein
Immunogen Species
Caenorhabditis elegans
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Involved in the control of stem cells and sex determination in the C.elegans hermaphrodite germline. May also play a role in the hermaphrodite germline proliferation and oogenesis. By binding to the 3'-UTR, represses phosphatase lip-1 expression in the distal part of the germline mitotic zone. Binds specifically to the regulatory region of fem-3 3'-UTR and mediates the sperm/oocyte switch. Negatively regulates gld-3 expression possibly by directly binding to two sites within the gld-3 isoform b 3'-UTR. Suppresses germline tumor formation by preventing the dedifferentiation of secondary spermatocytes.
Gene References into Functions
  1. findings show FBF-1 and FBF-2 have different effects on target mRNAs; FBF-2 prevents translation; FBF-2 activity depends on PGL-1; PGL-1 is required to localize FBF-2 to perinuclear P granules and for efficient binding of FBF-2 to its mRNA targets PMID: 22991439
  2. fbf-2 regulates the size of the mitotic region in helminth germ cells. PMID: 15525531
  3. Fbf-2 redundantly inhibits primary vulval cell fate specification in two distinct pathways acting in the soma and in the germline PMID: 16908630
Subcellular Location
Cytoplasm. Cytoplasmic granule.
Tissue Specificity
Expressed specifically in the germline (at protein level).
Database Links

KEGG: cel:CELE_F21H12.5

STRING: 6239.F21H12.5

UniGene: Cel.14869

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