fbl-1 Antibody

Code CSB-PA008452XA01CXY
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Product Details

Full Product Name
Rabbit anti-Caenorhabditis elegans fbl-1 Polyclonal antibody
Uniprot No.
Target Names
fbl-1
Alternative Names
fbl-1 antibody; fbln1 antibody; F56H11.1Fibulin-1 antibody
Raised in
Rabbit
Species Reactivity
Caenorhabditis elegans
Immunogen
Recombinant Caenorhabditis elegans fbl-1 protein
Immunogen Species
Caenorhabditis elegans
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Incorporated into fibronectin-containing matrix fibers. Plays a role in cell adhesion and migration along protein fibers within the extracellular matrix (ECM). Important for certain developmental processes and contributes to the supramolecular organization of ECM architecture, in particular to those of basement membranes.; Involved in regulating the shape and adhesion of cells in the developing pharynx, intestine, body-wall muscle and gonadal tissue. During gonadogenesis, regulates the width of gonads and the migration of distal tip cells (DTC). Together with type IV collagen let-2 and downstream of metalloprotease mig-17, recruits nidogen nid-1 to the gonad basement membrane thereby inducing basement membrane remodeling required for the directional migration of DTCs. Acts antagonistically with metalloprotease gon-1 to maintain optimal levels of type IV collagen emb-9 in the gonad basement membrane during gonadogenesis. Required for larval development.; Involved in the assembly of the flexible hemicentin-containing tracks found joining the pharynx and body-wall-muscle basement membranes.
Gene References into Functions
  1. A series of deletion constructs shows that EGF repeats 4 and 5 are required for the hemicentin-dependent assembly and function of fibulin-1D in native locations. PMID: 22981695
  2. There is a distinct developmental roles and hemicentin-dependent assembly for fibulin-1 splice variants PMID: 16120639
Subcellular Location
[Isoform a]: Secreted, extracellular space, extracellular matrix, basement membrane.
Protein Families
Fibulin family
Tissue Specificity
Expressed in head muscle cells, anterior and posterior intestinal cells. Isoform a: Expressed in male and hermaphrodite gonad, anterior and posterior intestine and pharyngeal basement membranes, body-wall muscle, GLR cells, uterine attachment and mechanos
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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