fib-1 Antibody

Code CSB-PA632535XA01CXY
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Product Details

Full Product Name
Rabbit anti-Caenorhabditis elegans fib-1 Polyclonal antibody
Uniprot No.
Target Names
fib-1
Alternative Names
fib-1 antibody; T01C3.7rRNA 2'-O-methyltransferase fibrillarin antibody; EC 2.1.1.- antibody; Histone-glutamine methyltransferase antibody
Raised in
Rabbit
Species Reactivity
Caenorhabditis elegans
Immunogen
Recombinant Caenorhabditis elegans fib-1 protein
Immunogen Species
Caenorhabditis elegans
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
S-adenosyl-L-methionine-dependent methyltransferase that has the ability to methylate both RNAs and proteins. Involved in pre-rRNA processing. Utilizes the methyl donor S-adenosyl-L-methionine to catalyze the site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA. Also acts as a protein methyltransferase by mediating methylation of 'Gln-105' of histone H2A (H2AQ105me), a modification that impairs binding of the FACT complex and is specifically present at 35S ribosomal DNA locus. Plays a role in modulation of nucleolus size most likely through regulating the ribosomal RNA (rRNA) pool.
Gene References into Functions
  1. Fibrillarin acts as a central node in a regulatory network engaged in imparting immunity against bacterial pathogens. PMID: 30190478
Subcellular Location
Nucleus, nucleolus. Nucleus, nucleoplasm.
Protein Families
Methyltransferase superfamily, Fibrillarin family
Database Links

KEGG: cel:CELE_T01C3.7

STRING: 6239.T01C3.7.1

UniGene: Cel.23500

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