FCA Antibody

Code CSB-PA515346XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) FCA Polyclonal antibody
Uniprot No.
Target Names
FCA
Alternative Names
FCA antibody; At4g16280 antibody; dl4180c antibody; dl4181c antibody; dl4182c antibody; FCAALL.331Flowering time control protein FCA antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana FCA protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Plays a major role in the promotion of the transition of the vegetative meristem to reproductive development. Plays a role in the regulation of flowering time in the autonomous flowering pathway by decreasing FLOWERING LOCUS C mRNA levels. Required for RNA-mediated chromatin silencing of a range of loci in the genome. Cotranscriptionally recognizes aberrant RNA and marks it for silencing. Controls alternative cleavage and polyadenylation on pre-mRNAs and antisense RNAs. Acts redundantly with FPA to prevent the expression of distally polyadenylated antisense RNAs at the FLC locus.
Gene References into Functions
  1. an FCA-mediated epigenetic route towards thermal adaptation of autotrophic development in plants PMID: 28454607
  2. regulation of YUC8 expression tunes down PIF4-induced architectural changes to achieve thermal adaptation of stem growth at high ambient temperature PMID: 25400039
  3. FCA plays a role in the induction of thermotolerance by triggering antioxidant accumulation under heat stress conditions. PMID: 25266977
  4. Data suggest that the FCA-miR172 regulon provides an adaptive strategy that fine tunes the onset of flowering under fluctuating ambient temperature conditions. PMID: 22431732
  5. Data show distinct layers of FCA regulation that involve sequences within the 5' region that regulate noncanonical translation initiation and alter the expression profile. PMID: 21075770
  6. FLC is repressed by genes of the autonomous promotion pathway. PMID: 16940039
  7. FCA does not bind ABA PMID: 19078995

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Subcellular Location
Nucleus.
Tissue Specificity
Constitutively expressed, but the negative feedback maintains the active isoform a low level throughout much of the plant, except in meristematic cells at a specific time in development.
Database Links

KEGG: ath:AT4G16280

STRING: 3702.AT4G16280.2

UniGene: At.219

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