fem-1 Antibody

Code CSB-PA321511XA01CXY
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Product Details

Full Product Name
Rabbit anti-Caenorhabditis elegans fem-1 Polyclonal antibody
Uniprot No.
Target Names
fem-1
Alternative Names
fem-1 antibody; isx-1 antibody; F35D6.1Sex-determining protein fem-1 antibody; Feminization of XX and XO animals protein 1 antibody
Raised in
Rabbit
Species Reactivity
Caenorhabditis elegans
Immunogen
Recombinant Caenorhabditis elegans fem-1 protein
Immunogen Species
Caenorhabditis elegans
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Substrate-recognition component of the cullin-RING-based CBC(fem-1) (Cul2-ElonginB-ElonginC) E3 ubiquitin-protein ligase complex of the DesCEND (destruction via C-end degrons) pathway, which recognizes a C-degron located at the extreme C terminus of target proteins, leading to their ubiquitination and degradation. The C-degron recognized by the DesCEND pathway is usually a motif of less than ten residues and can be present in full-length proteins, truncated proteins or proteolytically cleaved forms. Sex-determining protein; essential for the adoption of the male sexual fate in all tissues. The CBC(fem-1) complex mediates the ubiquitination and subsequent proteasomal degradation of tra-1. Promotes ubiquitination and degradation of cdl-1.
Gene References into Functions
  1. maternal fem-1 transcript is required to license expression of wild-type fem-1 allele in zygotic germline; defect in zygotic fem-1 expression is heritable, suggesting gene is subject to epigenetic silencing that is prevented by maternal fem-1 transcripts PMID: 21885785
  2. Results suggest that SEL-10-mediated proteolysis of FEM-1 and FEM-3 is required for normal hermaphrodite development. PMID: 15306688
Subcellular Location
Cytoplasm.
Protein Families
Fem-1 family
Database Links

KEGG: cel:CELE_F35D6.1

STRING: 6239.F35D6.1a

UniGene: Cel.5131

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