Product Details

Full Product Name

Rabbit anti-Mus musculus (Mouse) Fem1b Polyclonal antibody

Uniprot No.

Q9Z2G0

Target Names

Fem1b

Alternative Names

Fem1b antibody; F1aa antibody; Kiaa0396Protein fem-1 homolog B antibody; FEM1b antibody; FEM1-beta antibody; Fem-1-like death receptor-binding protein alpha antibody; Fem-1-like in apoptotic pathway protein alpha antibody; F1A-alpha antibody; mt-Fem antibody

Raised in

Rabbit

Species Reactivity

Mus musculus (Mouse)

Immunogen

Recombinant Mus musculus (Mouse) Fem1b protein

Immunogen Species

Mus musculus (Mouse)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Substrate-recognition component of a Cul2-RING (CRL2) E3 ubiquitin-protein ligase complex of the DesCEND (destruction via C-end degrons) pathway, which recognizes a C-degron located at the extreme C terminus of target proteins, leading to their ubiquitination and degradation. The C-degron recognized by the DesCEND pathway is usually a motif of less than ten residues and can be present in full-length proteins, truncated proteins or proteolytically cleaved forms. The CRL2(FEM1B) complex specifically recognizes proteins ending with -Gly-Leu-Asp-Arg, such as CDK5R1, leading to their ubiquitination and degradation. Also acts as a regulator of the reductive stress response by mediating ubiquitination of reduced FNIP1: in response to reductive stress, the CRL2(FEM1B) complex specifically recognizes a conserved Cys degron in FNIP1 when this degron is reduced, leading to FNIP1 degradation and subsequent activation of mitochondria to recalibrate reactive oxygen species (ROS). Promotes ubiquitination of GLI1, suppressing GLI1 transcriptional activator activity. Promotes ubiquitination and degradation of ANKRD37. Promotes ubiquitination and degradation of SLBP. Involved in apoptosis by acting as a death receptor-associated protein that mediates apoptosis. Also involved in glucose homeostasis in pancreatic islet. May also act as an adapter/mediator in replication stress-induced signaling that leads to the activation of CHEK1.

Gene References into Functions

Fem1b is a binding partner of Ankrd37, and their interaction resulted in Ankrd37 degradation through the ubiquitin-mediated proteolysis pathway. PMID: 21723927
Increased Fem1b is associated with intestinal neoplasia. PMID: 20952268
neither FEM1B nor PHTF1 are directly implicated in apoptosis in testis tissue PMID: 15601915
Fem1b is implicated in pancreatic islet function and insulin secretion PMID: 16024793
Fem1b may have a conserved role in the generation of sexual dimorphism through its interaction with Nkx3.1 in the developing prostate gland PMID: 18816836

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Subcellular Location

Cytoplasm. Nucleus.

Protein Families

Fem-1 family

Tissue Specificity

Expressed in pancreatic islets, within both beta cells and non-beta cells (at protein level). Highly expressed in adult testis; expressed in all types of spermatogonia. Also expressed in the prostate of neonatal mice.

Database Links

KEGG: mmu:14155

STRING: 10090.ENSMUSP00000034775

UniGene: Mm.24069

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Fem1b Antibody

Product Details

Customer Reviews and Q&A

Target Background