FIM5 Antibody

Code CSB-PA872264XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) FIM5 Polyclonal antibody
Uniprot No.
Target Names
FIM5
Alternative Names
FIM5 antibody; FIM2 antibody; At5g35700 antibody; MXH1.5Fimbrin-5 antibody; AtFIM5 antibody; Fimbrin-like protein 2 antibody; Fimbrin5 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana FIM5 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Cross-links actin filaments (F-actin) in a calcium independent manner. Induces the formation of actin bundles. Stabilizes and prevents F-actin depolymerization mediated by latrunculin B (LatB).
Gene References into Functions
  1. FIM5 organizes and maintains normal actin architecture in pollen grains and pollen tubes to fulfill double fertilization in plants. PMID: 29036437
  2. FIM5 decorates apical actin filaments and regulates their organization in order to drive polarized pollen tube growth. PMID: 27117336
  3. FIM4 generates both actin bundles and cross-linked actin filaments, whereas FIM5 only generates actin bundles. The distinct functions of FIM4 and FIM5 are clearly observed at single-filament resolution. PMID: 27261463
  4. Data suggest that FIMBRIN5 regulates actin dynamics and organization during pollen germination and tube growth via stabilizing actin filaments and organizing them into higher-order structures. [FIMBRIN5] PMID: 21098731
Subcellular Location
Cytoplasm, cytoskeleton.
Tissue Specificity
Expressed in mature pollen.
Database Links

KEGG: ath:AT5G35700

STRING: 3702.AT5G35700.1

UniGene: At.7495

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