FIT Antibody

Code CSB-PA611716XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) FIT Polyclonal antibody
Uniprot No.
Target Names
FIT
Alternative Names
FIT antibody; BHLH29 antibody; EN43 antibody; FIT1 antibody; FRU antibody; At2g28160 antibody; F24D13.5 antibody; Transcription factor FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION FACTOR antibody; Basic helix-loop-helix protein 29 antibody; AtbHLH29 antibody; bHLH 29 antibody; FER-LIKE REGULATOR OF IRON UPTAKE antibody; Transcription factor EN 43 antibody; Transcription factor Fe-DEFICIENCY INDUCED TRANSCRIPTION FACTOR 1 antibody; bHLH transcription factor bHLH029 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana FIT protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
Transcription factor. Essential protein involved in iron uptake responses. Regulates FRO2 at the level of mRNA accumulation and IRT1 at the level of protein accumulation. Confers enhanced iron mobilization responses at low iron supply.
Gene References into Functions
  1. 34 genes were robustly FIT-regulated in root and seedling samples and included 13 novel FIT-dependent genes. PMID: 27716045
  2. FIT interacts with ZAT12 linking iron deficiency and oxidative stress responses. PMID: 26556796
  3. FER-like iron deficiency-induced transcription factor functions as a direct or indirect modulator of protein levels of a subset of gene products under iron deficiency. PMID: 25951126
  4. Nitric oxide as a signal that promoted not only the activation of FIT but also its protein stability, independent of the transcriptional control by ethylene and Nitric oxide. PMID: 21972265
  5. bHLH100 and bHLH101 are key regulators of iron-deficiency responses independent of the master regulator FIT PMID: 22984573
  6. FIT is post-translationally regulated by iron deficiency, leading to a decrease in FIT protein stability mediated by proteasome-dependent degradation. PMID: 21426424
  7. We propose a new model for iron uptake in Arabidopsis where FRO2 and IRT1 are differentially regulated by FIT1 which encodes a putative transcription factor that regulates iron uptake responses in Arabidopsis thaliana [FIT1] PMID: 15539473
  8. AtbHLH29 encods a bHLH protein, which is a functional ortholog of the tomato FER gene which are proposed as transcriptional factors functioning in iron deficiency responses and iron uptake. [AtbHLH29] PMID: 16117851
  9. A proposed common name for At2g28160 in Arabidopsis, namely FIT=FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION FACTOR, is discussed. PMID: 17466530
  10. ferric-chelate reductase FRO2 and ferrous-transporter IRT1 are the targets of the three transcription factors and the transcription of FRO2 and IRT1 is directly regulated by a complex of FIT/AtbHLH38 or FIT/AtbHLH39. PMID: 18268542

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Subcellular Location
Nucleus.
Tissue Specificity
Expressed in roots and inflorescence, and to a lower extent, in leaves and stems. In roots, confined to the outer cell layers, specifically in the differentiation zone. Also detected in the endodermis and inner tissues of the central cylinder.
Database Links

KEGG: ath:AT2G28160

STRING: 3702.AT2G28160.1

UniGene: At.43510

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