FKH1 Antibody

Code CSB-PA340254XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FKH1 Polyclonal antibody
Uniprot No.
Target Names
FKH1
Alternative Names
FKH1 antibody; YIL131C antibody; Fork head protein homolog 1 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FKH1 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Protein A/G
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Transcription factor that regulates the expression of the CLB2 cluster of genes during the G2/M phase of the mitotic cell cycle. The CLB2 cluster of genes includes mitotic regulators such as CLB1, CLB2, CDC5 and CDC20 as well as SWI5 and ACE2, transcription factors required for the subsequent temporal wave of cell cycle regulated gene expression in the M/G1 phase interval. Involved in HMRa silencing. FKH1 and FKH2 associate with the coding regions of active genes and influence, in opposing ways, transcriptional elongation and termination, and coordinate early transcription elongation and pre-mRNA processing. Both FKH1 and FKH2 play a role as regulators of lifespan in collaboration with the anaphase-promoting complex (APC), likely through combined regulation of stress response, genomic stability, and cell cycle regulation. FKH1 and FKH2 function also in controlling yeast cell morphology by preventing preudohyphal growth. Acts as a rate-limiting replication origin activator via its interactin with the origin recognition complex (ORC). Plays a transcription-independent role in recombination donor preference during mating-type switching through binding to the recombination enhancer (RE), a 700-bp cis-acting element that controls recombination along the left arm of chromosome III.
Gene References into Functions
  1. Fkh1 and Fkh2 dimerization controls DNA replication timing and spatial organization of chromosomes. PMID: 28265091
  2. Spatial and directional arrangement of Fkh1/2 sites is crucial for efficient binding of the Fkh1 protein and for early firing of the origins. PMID: 28141805
  3. We have shown that the phosphothreonine binding motif of the FHA domain of Fkh1 plays a critical role in the regulation of donor preference during Saccharomyces cerevisiae mating-type switching PMID: 22496671
  4. This study establishes the FKH1 and FKH2 role as evolutionarily conserved regulators of lifespan in yeast and identifies the APC as a novel component of this mechanism PMID: 22438832
  5. Study shows that Fkh1 and Fkh2 regulate the initiation timing of most of the earliest origins in the yeast genome through a novel mechanism involving origin clustering in G1 phase. PMID: 22265405
  6. Disruption of fkh1-associated cell cycle transcription by oxidative stress PMID: 15371544
  7. Fkh1 binds to the recombination enhancer only in G(2), which contrasts with its cell cycle-independent binding to its other target promoters. PMID: 16809780
  8. These and other data supported a model in which FKH1 and CLB5 modulated Sir2-4 chromatin and late-origin firing through opposing regulation of a common pathway. PMID: 18045995

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Subcellular Location
Nucleus. Cytoplasm, cytosol.
Database Links

KEGG: sce:YIL131C

STRING: 4932.YIL131C

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