FKH2 Antibody

Code CSB-PA336909XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FKH2 Polyclonal antibody
Uniprot No.
Target Names
FKH2
Alternative Names
FKH2 antibody; YNL068C antibody; N2403 antibody; YNL2403CFork head protein homolog 2 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FKH2 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Transcription factor that regulates the expression of the CLB2 cluster of genes during the G2/M phase of the mitotic cell cycle. The CLB2 cluster of genes includes mitotic regulators such as CLB1, CLB2, CDC5 and CDC20 as well as SWI5 and ACE2, transcription factors required for the subsequent temporal wave of cell cycle regulated gene expression in the M/G1 phase interval. Involved in HMRa silencing. FKH1 and FKH2 associate with the coding regions of active genes and influence, in opposing ways, transcriptional elongation and termination, and coordinate early transcription elongation and pre-mRNA processing. Both FKH1 and FKH2 play a role as regulators of lifespan in collaboration with the anaphase-promoting complex (APC), likely through combined regulation of stress response, genomic stability, and cell cycle regulation. FKH1 and FKH2 function also in controlling yeast cell morphology by preventing preudohyphal growth. Acts as a rate-limiting replication origin activator via its interactin with the origin recognition complex (ORC).
Gene References into Functions
  1. Disruption of fkh2-associated cell cycle transcription by oxidative stress PMID: 15371544
  2. Fkh2p is a target of cyclin-dependent protein kinases, and phosphorylation of Fkh2p promotes interactions between Fkh2p and the coactivator Ndd1p PMID: 15509804
Subcellular Location
Nucleus. Cytoplasm, cytosol.
Database Links

KEGG: sce:YNL068C

STRING: 4932.YNL068C

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