fkh2 Antibody

Code CSB-PA526440XA01SXV
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Product Details

Full Product Name
Rabbit anti-Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) fkh2 Polyclonal antibody
Uniprot No.
Target Names
fkh2
Alternative Names
fkh2 antibody; SPBC16G5.15cFork head protein homolog 2 antibody
Raised in
Rabbit
Species Reactivity
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Immunogen
Recombinant Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) fkh2 protein
Immunogen Species
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Required for promoter sequence element PCB-driven, M-phase-specific transcription. Acts as a transcriptional activator with a role in the regulation of mitosis. Binds, cooperatively with mcm1, the CLB cluster regulatory elements throughout the cell cycle. Regulates the periodic transcription of cdc15 and spo12. Required for the correct timing, positioning and contraction of the division septum.
Gene References into Functions
  1. Fkh2 marks promoters for Mei4 binding during meiosis. PMID: 26804917
  2. Fkh2 regulates not only the onset of mitotic transcription but also the correct timing of mitotic entry via effects on the Wee1 kinase PMID: 22451489
  3. Vegetative expression of mid-meiotic genes is repressed at least two independent ways: antisense transcription and Fkh2 repression. PMID: 22238674
  4. The forkhead transcription factor Fkh2 regulates the periodic expression of cdc15+ & spo12+ in the M & G1 phases of the cell division cycle. It also regulates several cell cycle-dependent processes, including septum formation & mitotic spindle function. PMID: 15302827
  5. activation of the Fkh2p and Sep1p forkhead transcription factors triggers mitotic gene transcription in fission yeast. PMID: 15509866
  6. Deletion of fkh2 (similar to FKH2 of S. cerevisiae) reduced the growth rate and caused an extension of cell length due to delayed G2-to-M transition. PMID: 15777722

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Subcellular Location
Nucleus.
Database Links
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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