Product Details

Full Product Name

Rabbit anti-Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) clp1 Polyclonal antibody

Uniprot No.

Q9P7H1

Target Names

clp1

Alternative Names

clp1 antibody; flp1 antibody; SPAC1782.09c antibody; Tyrosine-protein phosphatase CDC14 homolog antibody; EC 3.1.3.48 antibody; CDC fourteen-like phosphatase 1 antibody

Raised in

Rabbit

Species Reactivity

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Immunogen

Recombinant Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) clp1 protein

Immunogen Species

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Protein phosphatase which antagonizes mitotic cyclin-dependent kinase cdc2, the inactivation of which is essential for exit from mitosis. To access its substrates, is released from nucleolar sequestration during mitosis. Plays an essential in coordinating the nuclear division cycle with cytokinesis through the cytokinesis checkpoint. Involved in chromosome segregation, where it is required for meiosis I spindle dissambly as well as for establishing two consecutive chromosome segregation phases. Allows damaged actomyosin rings to be maintained to facilitate completion of cell division in response to minor perturbation of the cell division machinery. Dephosphorylates the mitotic inducer cdc25 for its rapid degradation. Down-regulation of cdc25 activity ensures a prompt inactivation of mitotic cdc2 complexes to trigger cell division. Dephosphorylates also cdc2-phosphorylated nsk1, allowing nsk1-binding to kinetochores and spindle. Dephosphorylates ase1, which is essential for spindle midzone assembly and for continuous extension of the anaphase spindle. Tethered to the contractile ring by mid1, where it dephosphorylates cdc15.

Gene References into Functions

Sal3, a nuclear beta-importin, is the sole karyopherin required for Clp1 nucleoplasmic shuttling. PMID: 23297348
Relocalization of Clp1 during genotoxic stress is governed by complex phosphoregulation. PMID: 22918952
Clp1p is required to dephosphorylate, destabilize, and inactivate Cdc25p at the end of mitosis PMID: 14765109
Clp1p/Flp1p-dependent cytokinesis checkpoint provides a previously unrecognized cell survival advantage when the cell division apparatus is mildly perturbed PMID: 15265986
hCdc14A and hCdc14B have functional homology to S. pombe Cdc25 and flp1 PMID: 15911625
Clp1 plays distinct roles in chromosome segreagation when localized in the nucleus, and cytokinesis, in the nucleolus. PMID: 16085490
These findings point to a simple regulatory circuit that couples Cdk1 activation with its inactivation mediated through phosphorylation-dependent regulation of Clp1/Flp1 phosphatase activity. PMID: 16950131
Thus Flp1p appears to have an important role in integrating HECT- and RING-type ubiquitin ligases in cell cycle control. PMID: 18418059
Clp1 is maintained in the cytoplasm by Sid2. Loss of Clp1 from the cytoplasm in telophase renders cells sensitive to perturbation of the actomyosin ring but does not affect other Clp1 functions. PMID: 18951025

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Subcellular Location

Nucleus, nucleolus. Cytoplasm, cytoskeleton, microtubule organizing center, spindle pole body. Cell septum. Note=Localizes to kinetochores in prometaphase. Cytoplasmic retention is mediated through the binding of rad24. Tethered to the contractile ring by mid1.

Protein Families

Protein-tyrosine phosphatase family, Non-receptor class CDC14 subfamily

Database Links

KEGG: spo:SPAC1782.09c

STRING: 4896.SPAC1782.09c.1

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clp1 Antibody

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