Product Details

Purity

>85% (SDS-PAGE)

Target Names

clp1

Uniprot No.

Q9P7H1

Alternative Names

clp1; flp1; SPAC1782.09c; Tyrosine-protein phosphatase CDC14 homolog; EC 3.1.3.48; CDC fourteen-like phosphatase 1

Species

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Protein phosphatase which antagonizes mitotic cyclin-dependent kinase cdc2, the inactivation of which is essential for exit from mitosis. To access its substrates, is released from nucleolar sequestration during mitosis. Plays an essential in coordinating the nuclear division cycle with cytokinesis through the cytokinesis checkpoint. Involved in chromosome segregation, where it is required for meiosis I spindle dissambly as well as for establishing two consecutive chromosome segregation phases. Allows damaged actomyosin rings to be maintained to facilitate completion of cell division in response to minor perturbation of the cell division machinery. Dephosphorylates the mitotic inducer cdc25 for its rapid degradation. Down-regulation of cdc25 activity ensures a prompt inactivation of mitotic cdc2 complexes to trigger cell division. Dephosphorylates also cdc2-phosphorylated nsk1, allowing nsk1-binding to kinetochores and spindle. Dephosphorylates ase1, which is essential for spindle midzone assembly and for continuous extension of the anaphase spindle. Tethered to the contractile ring by mid1, where it dephosphorylates cdc15.

Gene References into Functions

Sal3, a nuclear beta-importin, is the sole karyopherin required for Clp1 nucleoplasmic shuttling. PMID: 23297348
Relocalization of Clp1 during genotoxic stress is governed by complex phosphoregulation. PMID: 22918952
Clp1p is required to dephosphorylate, destabilize, and inactivate Cdc25p at the end of mitosis PMID: 14765109
Clp1p/Flp1p-dependent cytokinesis checkpoint provides a previously unrecognized cell survival advantage when the cell division apparatus is mildly perturbed PMID: 15265986
hCdc14A and hCdc14B have functional homology to S. pombe Cdc25 and flp1 PMID: 15911625
Clp1 plays distinct roles in chromosome segreagation when localized in the nucleus, and cytokinesis, in the nucleolus. PMID: 16085490
These findings point to a simple regulatory circuit that couples Cdk1 activation with its inactivation mediated through phosphorylation-dependent regulation of Clp1/Flp1 phosphatase activity. PMID: 16950131
Thus Flp1p appears to have an important role in integrating HECT- and RING-type ubiquitin ligases in cell cycle control. PMID: 18418059
Clp1 is maintained in the cytoplasm by Sid2. Loss of Clp1 from the cytoplasm in telophase renders cells sensitive to perturbation of the actomyosin ring but does not affect other Clp1 functions. PMID: 18951025

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Subcellular Location

Nucleus, nucleolus. Cytoplasm, cytoskeleton, microtubule organizing center, spindle pole body. Cell septum. Note=Localizes to kinetochores in prometaphase. Cytoplasmic retention is mediated through the binding of rad24. Tethered to the contractile ring by mid1.

Protein Families

Protein-tyrosine phosphatase family, Non-receptor class CDC14 subfamily

Database Links

KEGG: spo:SPAC1782.09c

STRING: 4896.SPAC1782.09c.1

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Recombinant Schizosaccharomyces pombe Tyrosine-protein phosphatase CDC14 homolog (clp1), partial

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