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bin Antibody

Datasheet
Code CSB-PA131083XA01DLU
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Product Details

Full Product Name
Rabbit anti-Drosophila melanogaster (Fruit fly) bin Polyclonal antibody
Uniprot No.
Q9VS05
Target Names
bin
Alternative Names
bin antibody; FoxF antibody; CG18647Forkhead box protein biniou antibody; Forkhead box protein FoxF antibody; DmFoxF antibody
Raised in
Rabbit
Species Reactivity
Drosophila melanogaster (Fruit fly)
Immunogen
Recombinant Drosophila melanogaster (Fruit fly) bin protein
Immunogen Species
Drosophila melanogaster (Fruit fly)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
ELISA Protocol
Troubleshooting and FAQs
Antibody FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
Component of a regulatory network controlling visceral mesoderm development and midgut morphogenesis. Transcriptional regulator involved in the activation of a large number of genes in the visceral mesoderm including betaTub60D, dpp and Hand. Binds to and regulates a number of enhancers driving expression in the visceral mesoderm in a temporally and spatially restricted manner. Also to binds to enhancers cooperatively with activators, such as bap or HLH54F, to coregulate expression of shared target genes in the visceral mesoderm. Binds to the Ndg enhancer and drives expression of Ndg in the late visceral musculature. May be involved in the transcriptional regulation of wupA in the visceral mesoderm. Plays an indirect role in the later stages of salivary gland positioning.
Gene References into Functions
  1. In stage 10-11 embryos, the DmFoxF protein is detected into the nuclei of cells of the presumptive visceral mesoderm. It localizes at the segmental cell clusters of the mesoderm, which will eventually develop to surround the midgut endoderm. PMID: 11804790
  2. Biniou regulates hand expression by direct binding to a 300 bp sequence element, located within the 3rd intron of the hand gene PMID: 17511863
Subcellular Location
Nucleus.
Tissue Specificity
In embryo, expressed in all types of visceral muscles and their progenitors (at protein level). In late stage 10 embryo, expressed in the caudal visceral mesoderm and trunk and hindgut visceral mesoderm progenitors.
Database Links

KEGG: dme:Dmel_CG18647

STRING: 7227.FBpp0076609

UniGene: Dm.5684

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