fpg1 Antibody

Code CSB-PA966280XA01FSG
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Product Details

Full Product Name
Rabbit anti-Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) fpg1 Polyclonal antibody
Uniprot No.
Target Names
fpg1
Alternative Names
fpg1 antibody; mutM antibody; Rv2924c antibody; MTCY338.13cFormamidopyrimidine-DNA glycosylase 1 antibody; Fapy-DNA glycosylase 1 antibody; EC 3.2.2.23 antibody; DNA-(apurinic or apyrimidinic site) lyase MutM 1 antibody; AP lyase MutM 1 antibody; EC 4.2.99.18 antibody
Raised in
Rabbit
Species Reactivity
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Immunogen
Recombinant Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) fpg1 protein
Immunogen Species
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized purines, such as 7,8-dihydro-8-oxoguanine (8-oxoG) when paired with C, G or T, as well as methyl-faPy (formanidopyrimidine residues) in poly(dG-dC) and spiroiminodihydantoin:C base pairs. Unlike its E.coli ortholog has no activity on 8-oxoG:A. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates. Cleaves ssDNA containing an AP site. Complements the H(2)O(2) sensitivity of an M.smegmatis fpg disruption mutant; upon expression in M.smegmatis excises 8-oxoG from dsDNA.
Gene References into Functions
  1. The protein encoded by Mycobacterium tuberculosis Rv2924c removed formamidopyrimidine and 5-hydroxycytosine lesions, as well as 8-oxo-7, 8-dihydroguanine (8oxoG) opposite to C, T and G. PMID: 19496823
Protein Families
FPG family
Database Links

KEGG: mtu:Rv2924c

STRING: 83332.Rv2924c

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