Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) FREE1 Polyclonal antibody

Uniprot No.

Q9ASS2

Target Names

FREE1

Alternative Names

FREE1 antibody; FYVE1 antibody; At1g20110 antibody; T20H2.10Protein FREE1 antibody; FYVE domain protein required for endosomal sorting 1 antibody; FYVE domain-containing protein 1 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana FREE1 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Endosomal sorting complex required for transport (ESCRT) component regulating multivesicular body (MVB) protein sorting and plant growth. Required for the formation of intra-luminal vesicles (ILVs)in MVBs. Binds to phosphatidylinositol-3-phosphate (PI3P) and ubiquitin. Controls IRT1 recycling to the plasma membrane and impacts the polar delivery of this transporter to the outer plasma membrane domain. Regulates ubiquitin-dependent membrane protein degradation, vacuolar transport, autophagy, and vacuole biogenesis. ESCRT component that binds ubiquitin and regulates vacuolar sorting of proteins. Attenuates abscisic acid (ABA) signaling through RSL1-triggered degradation of the ABA receptors PYR1 and PYL4. Interacts with PYL4 and PYR1, and delivers the ubiquitinated ABA receptors as cargo to the vacuolar degradation pathway. In response to ABA, is phosphorylated by SnRK2 kinases which mediate FREE1 nuclear import. In the nucleus, interacts with the ABA-responsive transcription factors ABF4 and ABI5 to reduce their ability to bind to their cis-regulatory sequences of downstream genes, thus leading to transcriptional inhibition of ABA signaling pathway. Negatively regulates salt stress tolerance via a negative feedback loop involving ABA signaling pathway.

Gene References into Functions

FYVE1 is essential for plant growth and development and place FYVE1 as a key regulator of intracellular trafficking and vacuole biogenesis. PMID: 25699591
FREE1 binds to phosphatidylinositol-3-phosphate (PI3P) and ubiquitin and specifically interacts with Vps23 via PTAP-like tetrapeptide motifs to be incorporated into the ESCRT-I complex. PMID: 25438943
In addition to regulating MVB biogenesis, the ESCRT component FYVE domain protein required for endosomal sorting 1 (FREE1) also plays dual roles in vacuolar protein transport and autophagic degradation. [FREE1] PMID: 25624505

Subcellular Location

Cytoplasm. Prevacuolar compartment membrane; Peripheral membrane protein. Late endosome. Endosome, multivesicular body. Nucleus.

Tissue Specificity

Ubiquitous. Lowest expression in mature seeds.

Database Links

KEGG: ath:AT1G20110

STRING: 3702.AT1G20110.1

UniGene: At.15444

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FREE1 Antibody

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Target Background