FTSI Antibody

^{Rare Species}

Datasheet

Code	CSB-PA364923XA01ENV
Size	US$299
Image	Western Blot Positive WB detected in Recombinant protein All lanes: FTSI antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 32.4 kDa Observed band size: 38 kDa
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Target Background

Product Details

Full Product Name

Rabbit anti-Escherichia coli (strain K12) ftsI Polyclonal antibody

Uniprot No.

P0AD68

Target Names

ftsI

Alternative Names

ftsI antibody; pbpB antibody; b0084 antibody; JW0082Peptidoglycan D,D-transpeptidase FtsI antibody; EC 3.4.16.4 antibody; Essential cell division protein FtsI antibody; Murein transpeptidase antibody; Penicillin-binding protein 3 antibody; PBP-3 antibody; Peptidoglycan synthase FtsI antibody

Raised in

Rabbit

Species Reactivity

Escherichia coli (strain K12)

Immunogen

Recombinant Escherichia coli (strain K12) FTSI protein

Immunogen Species

Escherichia coli (strain K12)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Protein A/G

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB

Protocols

ELISA Protocol
Western Blotting(WB) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

The recombinant Escherichia coli (strain K12) FTSI protein is introduced into a rabbit to stimulate the production of antibodies that target this antigen. An additional injection is given to boost antibody production. The resulting FTSI polyclonal antibodies in the rabbit's serum are then collected and purified using protein A/G. Rigorous testing in ELISA and WB assays has confirmed that the FTSI antibody specifically recognizes Escherichia coli (strain K12) FTSI protein.

The main function of Escherichia coli (strain K12) FTSI protein, also known as penicillin-binding protein 3 (PBP3), is to catalyze the final step in bacterial cell wall synthesis. FTSI is a transpeptidase enzyme that forms cross-links between the peptidoglycan strands of the bacterial cell wall, providing structural integrity to the cell. This protein is essential for cell division in E. coli and plays a crucial role in maintaining cell shape and viability.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Essential cell division protein that catalyzes cross-linking of the peptidoglycan cell wall at the division septum. Required for localization of FtsN.

Gene References into Functions

A beta-lactam MIC of 8 mug/ml was reported for the three combinations against one Escherichia coli isolate with an unusual TIPY insertion following Tyr344 in penicillin-binding protein 3 (PBP 3) as the result of gene duplication. PMID: 28559260
In this work the authors show direct interactions between FtsW and PBP3 in vivo and in vitro by FRET (Forster resonance energy transfer) and co-immunoprecipitation experiments. PMID: 20847002
Alanine-scanning mutagenesis of the Transmembrane helix identified several residues important for septal localization. PMID: 15601716
direct interaction between a class B PBP (PBP3) and a class A PBP (PBP1B) in vitro and in vivo, indicating that different murein synthases might act in concert to enlarge the murein sacculus during cell division PMID: 16803586
FtsI is associated in a hierarchical set of protein complexes and its assembly can occur independently of 'upstream' proteins. PMID: 16824093
Data show that PBP3 interact with MtgA and help MtgA's role in peptidoglycan assembly during the cell cycle. PMID: 18165305

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Subcellular Location

Cell inner membrane; Single-pass membrane protein; Periplasmic side.

Protein Families

Transpeptidase family, FtsI subfamily

Database Links

KEGG: ecj:JW0082

STRING: 316385.ECDH10B_0066

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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Address

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FTSI Antibody

Product Details

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Customer Reviews and Q&A

Target Background