Product Details

Full Product Name

Rabbit anti-Escherichia coli (strain K12) ftsY Polyclonal antibody

Uniprot No.

P10121

Target Names

ftsY

Alternative Names

ftsY antibody; b3464 antibody; JW3429Signal recognition particle receptor FtsY antibody; SRP receptor antibody

Raised in

Rabbit

Species Reactivity

Escherichia coli (strain K12)

Immunogen

Recombinant Escherichia coli (strain K12) ftsY protein

Immunogen Species

Escherichia coli (strain K12)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Acts as a receptor for the complex formed by the signal recognition particle (SRP) and the ribosome-nascent chain (RNC). Interaction with SRP-RNC leads to the transfer of the RNC complex to the Sec translocase for insertion into the membrane, the hydrolysis of GTP by both Ffh and FtsY, and the dissociation of the SRP-FtsY complex into the individual components.

Gene References into Functions

FtsY can target the membrane co-translationally. PMID: 25653387
analysis of the ribosome-SRP-FtsY cotranslational targeting complex in the closed state PMID: 25775537
Data show that phospholipid binding drives important conformational rearrangements that activate the bacterial SRP receptor FtsY and the SRP-FtsY complex. PMID: 20733058
FtsY directly interacts with the SecY translocon. PMID: 15815684
Deletion experiments showed that this helix is essential for FtsY function in vivo, thus offering clear evidence for the functionally important, physiologically relevant interaction of FtsY with lipids. PMID: 17726012
In conclusion, we propose that the SRP receptor, FtsY has two distinct and separable roles in (i) mediating membrane targeting and docking of ribosomes and (ii) promoting their productive release from the docking site. PMID: 17726013
membrane binding and function of FtsY are in part regulated by proteolytic cleavage of the conserved 14-amino-acid motif. PMID: 18281057
the SRP and SR GTPases, without recruiting external regulatory factors, constitute a self-sufficient system that provides exquisite spatial and temporal control of a complex cellular process. PMID: 19174514
The data reveal that the two consecutive lipid-binding helices of FtsY can provide a membrane contact that does not differ significantly in stability from that provided by a transmembrane domain. PMID: 19414018

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Subcellular Location

Cell inner membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm.

Protein Families

GTP-binding SRP family, FtsY subfamily

Database Links

KEGG: ecj:JW3429

STRING: 316385.ECDH10B_3638

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ftsY Antibody

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