FUM2 Antibody

Code CSB-PA887762XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) FUM2 Polyclonal antibody
Uniprot No.
Target Names
FUM2
Alternative Names
FUM2 antibody; At5g50950 antibody; K3K7.11Fumarate hydratase 2 antibody; AtFUM2 antibody; Fumarase 2 antibody; EC 4.2.1.2 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana FUM2 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Cytosolic fumarate hydratase that catalyzes the reversible stereospecific interconversion of fumarate to L-malate. Catalyzes the dehydration of L-malate to fumarate in the cytosol: required for the massive fumarate accumulation during the day in plants grown under high nitrogen. Also required for acclimation of photosynthesis to cold: acts by mediating accumulation of fumarate at low temperature, leading to reduce accumulation of phosphorylated sugars.
Gene References into Functions
  1. Mutants lacking fumarase FUM2 show marked differences in their response to cold, with contrasting responses both in terms of metabolite concentrations and gene expression. PMID: 27440755
  2. FUM2 is required for the accumulation of fumarate in leaves, which is in turn required for rapid nitrogen assimilation and growth on high nitrogen. PMID: 20202172
Subcellular Location
Cytoplasm, cytosol.
Protein Families
Class-II fumarase/aspartase family, Fumarase subfamily
Database Links

KEGG: ath:AT5G50950

STRING: 3702.AT5G50950.1

UniGene: At.6963

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