FUS3 Antibody

Code CSB-PA888843XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) FUS3 Polyclonal antibody
Uniprot No.
Target Names
FUS3
Alternative Names
FUS3 antibody; At3g26790 antibody; MDJ14.4B3 domain-containing transcription factor FUS3 antibody; Protein FUSCA3 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana FUS3 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Transcription regulator involved in gene regulation during late embryogenesis. Its expression to the epidermis is sufficient to control foliar organ identity by regulating positively the synthesis abscisic acid (ABA) and negatively gibberellin production. Negatively regulates TTG1 in the embryo. Positively regulates the abundance of the ABI3 protein in the seed. Cooperates with KIN10 to regulate developmental phase transitions and lateral organ development and act both as positive regulators of abscisic acid (ABA) signaling during germination.
Gene References into Functions
  1. AIP2 targets FUS3 for degradation and plays a role in cotyledon development and flowering time in Arabidopsis. PMID: 28369580
  2. FUS3 phosphorylation and SnRK1 are required for embryogenesis and integration of environmental cues to ensure the survival of the progeny. PMID: 28922765
  3. LEAFY COTYLEDON2 (LEC2) was identified as an interacting factor of FUS3, and demonstrated that these two homologous B3 transcription factors interact to bind to the auxin biosynthesis gene YUCCA4 (YUC4) and synergistically activate its transcription during lateral roots formation. PMID: 27878992
  4. TT2 is directly bound to the regulatory region of FUSCA3, and mediates the expression of numerous genes in the fatty acid biosynthesis pathway. PMID: 24397827
  5. Examination of direct targets of FUS3 reveals that FUS3 acts primarily or exclusively as a transcriptional activator. PMID: 23314941
  6. Activation of FUS3 after germination dampens the expression of genes involved in the biosynthesis and response to the plant hormone ethylene, whereas a loss-of-function fus3 mutant shows many phenotypes consistent with increased ethylene signaling. PMID: 22348746
  7. FUS3 contributes to the delay of seed germination at high temperature. PMID: 22279962
  8. The genes positively controlled by FUS3 are not confined to previously known seed maturation-related genes and include those involved in the production of secondary metabolites and those involved in primary metabolism. PMID: 21045071
  9. The C-terminal domain is required for normal FUS3 function and sensitivity to abscisic acid and gibberellic acid, and negatively regulates mRNA and protein levels. PMID: 20663088
  10. In this study, it was shown that gibberellin (GA) hormone biosynthesis is regulated by LEC2 and FUS3 pathways. PMID: 15516508
  11. At2S3 activation by FUS3 was rapid but CRC induction by FUS3 with abscissic acid[ABA] and by ABA followed by presence of FUS3 took longer, suggesting involvement of an indirect mechanism requiring the synthesis of intermediate regulatory factor(s) PMID: 15695463
  12. Total repression of embryogenic potential was observed in double (lec1 lec2, lec1 fus3, lec2 fus3) and triple (fus3 lec1 lec2) mutants. PMID: 16034595
  13. Results show that Significant differences in histone modifications at the phas promoter were mediated by FUS3 and PvALF, suggesting that they function through different epigenetic mechanisms. PMID: 18038114
  14. FUS3 function is restricted to the acquisition of embryo-dependent seed dormancy, the determination of cotyledonary cell identity, and the synthesis and accumulation of storage compounds. PMID: 18343361

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Subcellular Location
Nucleus.
Tissue Specificity
Expressed in cotyledons and hypocotyls.
Database Links

KEGG: ath:AT3G26790

STRING: 3702.AT3G26790.1

UniGene: At.6029

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