FUS3 Antibody

Code CSB-PA322895XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUS3 Polyclonal antibody
Uniprot No.
Target Names
FUS3
Alternative Names
FUS3 antibody; DAC2 antibody; YBL016W antibody; YBL03.21 antibody; YBL0303Mitogen-activated protein kinase FUS3 antibody; MAP kinase FUS3 antibody; EC 2.7.11.24 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FUS3 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Together with closely related KSS1, FUS3 is the final kinase in the signal transduction cascade regulating activation/repression of the mating and filamentation pathways, induced by pheromone and nitrogen/carbon limitation, respectively. Phosphorylated FUS3 activates the mating but suppresses the filamentation pathway, whereas activated KSS1 activates both pathways. Pheromone-activated FUS3 functions by inhibiting the binding of the transcriptional activator STE12 to filamentation specific genes while inducing its binding to and activity at mating specific genes. Non-activated FUS3 has a repressive effect on STE12 transcriptional activity. KSS1 can partially compensate for the lack of FUS3 but mating efficiency is reduced and the filamentation program is partially activated upon pheromone signaling. FUS3 phosphorylates STE7, STE5, FAR1, DIG1, DIG2 and STE12.
Gene References into Functions
  1. Fus3 inhibits filamentous growth during mating by degrading Tec1, a cofactor of Ste12 for the expression of filamentation genes. PMID: 15620356
  2. This analysis documents direct phosphorylation of yeast G protein signaling regulator Sst2 by Fus3, and validates the prevailing model for MAP kinase recognition using a native protein substrate in vivo and small synthetic peptides tested in vitro. PMID: 15924435
  3. Results examine the role of docking interactions in determining connectivity of the yeast MAPKs Fus3 and Kss1. PMID: 16364919
  4. Ste5 scaffold has a more active role in the mating pathway; a fragment of Ste5 allosterically activated autophosphorylation of Fus3 PMID: 16424299
  5. Fus3 induces polarized growth in G1 phase cells by down-regulating Ste5 recruitment and by inhibiting Cln/Cdc28 kinase, which prevents basal recruitment of Ste5, Cdc42-mediated asymmetry, and mating morphogenesis. PMID: 18256288
  6. Fus3-triggered Tec1 degradation modulates mating transcriptional output during the pheromone response. PMID: 18682702
  7. Peaks in Fus3 activation correlated with periodic rounds of cell morphogenesis, with each peak preceding the formation of an additional mating projection. PMID: 18976914
  8. the mitogen-activated protein kinase Fus3 mediates fast-acting negative feedback that adjusts the dose response of the downstream system response to match the dose response of receptor-ligand binding PMID: 19079053

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Subcellular Location
Nucleus. Cytoplasm. Periplasm. Note=FUS3 shuttles rapidly between the cytoplasm and the nucleus independent of pheromone treatment or FUS3 phosphorylation level. Activated FUS3 translocates rapidly to the nucleus.
Protein Families
Protein kinase superfamily, CMGC Ser/Thr protein kinase family, MAP kinase subfamily
Database Links

KEGG: sce:YBL016W

STRING: 4932.YBL016W

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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