Product Details

Full Product Name

Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FZO1 Polyclonal antibody

Uniprot No.

P38297

Target Names

FZO1

Alternative Names

FZO1 antibody; YBR179C antibody; YBR1241Mitofusin FZO1 antibody; EC 3.6.5.- antibody; Transmembrane GTPase FZO1 antibody

Raised in

Rabbit

Species Reactivity

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Immunogen

Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) FZO1 protein

Immunogen Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Essential transmembrane GTPase, which mediates mitochondrial fusion. Fusion proceeds through several steps; first mitochondria are tethered together, then brought into close contact, followed by the formation of a docking ring around contact areas, and finally membrane fusion. Fusion of mitochondria occurs in many cell types and constitutes an important step in mitochondrial morphology, which is balanced between fusion and fission, mediated by FZO1 and DNM1, respectively. Functions antagonistically with DNM1. Probably acts by forming membrane contact sites that mediate mitochondrial membrane fusion. Mitochondrial docking and fusion requires GTP hydrolysis. Mitochondrial fusion promotes also increased lifespan.

Gene References into Functions

The Saccharomyces cerevisiae Fzo1 and animal Mitofusin proteins are highly diverged from one another and lack strong sequence similarity. PMID: 29212658
The results indicated that two different Ubp3 and Ubx2 complexes independently regulate mitochondrial fusion and Fzo1 turnover. PMID: 29924334
Results identify diverse effects of CMT2A mutations, including a possible role for Mfn2 ubiquitylation and degradation in CMT2A pathogenesis, and provide evidence for a link between Fzo1 GTP hydrolysis, ubiquitylation, and mitochondrial fusion. PMID: 19812251
homotypic trans interactions of Fzo1 were required to promote the fusion of mitochondrial outer membranes PMID: 15297626
proteasomal degradation of Fzo1 in response to the mating pheromone may have a role in mediating the remodeling of the mitochondrial network during the process of mating PMID: 15760898
Fzo1 oligomer domain interactions are essential for mitochondrial fusion PMID: 16624808
This study provides a framework for developing an understanding of the function of Mdm30p-mediated Fzo1p degradation in the multistep process of mitochondrial fusion. PMID: 18353967

Hide All

Subcellular Location

Mitochondrion outer membrane; Multi-pass membrane protein.

Protein Families

TRAFAC class dynamin-like GTPase superfamily, Dynamin/Fzo/YdjA family, Mitofusin subfamily

Database Links

KEGG: sce:YBR179C

STRING: 4932.YBR179C

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：

Code	CSB-PA339948XA01SVG
Size	Enquire
Have Questions?	Leave a Message or Start an on-line Chat

FZO1 Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background