gapA Antibody

Code CSB-PA359273XA01ENV
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Product Details

Full Product Name
Rabbit anti-Escherichia coli (strain K12) gapA Polyclonal antibody
Uniprot No.
Target Names
gapA
Alternative Names
gapA antibody; b1779 antibody; JW1768Glyceraldehyde-3-phosphate dehydrogenase A antibody; GAPDH-A antibody; EC 1.2.1.12 antibody; NAD-dependent glyceraldehyde-3-phosphate dehydrogenase antibody
Raised in
Rabbit
Species Reactivity
Escherichia coli (strain K12)
Immunogen
Recombinant Escherichia coli (strain K12) gapA protein
Immunogen Species
Escherichia coli (strain K12)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Catalyzes the oxidative phosphorylation of glyceraldehyde 3-phosphate (G3P) to 1,3-bisphosphoglycerate (BPG) using the cofactor NAD. The first reaction step involves the formation of a hemiacetal intermediate between G3P and a cysteine residue, and this hemiacetal intermediate is then oxidized to a thioester, with concomitant reduction of NAD to NADH. The reduced NADH is then exchanged with the second NAD, and the thioester is attacked by a nucleophilic inorganic phosphate to produce BPG.
Gene References into Functions
  1. promoter gapA P1 is a very efficient promoter sharing common properties with both extended -10 and non-extended -10 promoters PMID: 15250823
  2. Data indicate that exported glyceraldehyde-3-phosphate dehydrogenase may act as a virulence factor which could contribute to bacterial pathogenesis. PMID: 17449317
  3. The authors report that GAPDH of these pathogens is ADP-ribosylated either in the cytoplasm or in the extracellular medium. GAPDH catalyzes its own modification, which involves Cys-149 at the active site. PMID: 19784925
Subcellular Location
Cytoplasm.
Protein Families
Glyceraldehyde-3-phosphate dehydrogenase family
Database Links
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