GAPCP1 Antibody

Code CSB-PA238688XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) GAPCP1 Polyclonal antibody
Uniprot No.
Target Names
GAPCP1
Alternative Names
GAPCP1 antibody; At1g79530 antibody; T8K14.5Glyceraldehyde-3-phosphate dehydrogenase GAPCP1 antibody; chloroplastic antibody; EC 1.2.1.12 antibody; Glyceraldehyde-3-phosphate dehydrogenase of plastid 1 antibody; NAD-dependent glyceraldehydephosphate dehydrogenase chloroplastic 1 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana GAPCP1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Involved in plastidial glycolytic pathway and plays a specific role in glycolytic energy production in non-green plastids and chloroplasts. Essential for breakdown of starch to form sucrose for export to non-photosynthetic tissues, and to generate primary metabolites for anabolic pathways such as fatty acid and amino acid synthesis. Plays an important role in plant development by providing substrates for the phosphorylated pathway of serine biosynthesis in roots. Plays a crucial role in pollen development. Functionally redundant with GAPCP2.
Gene References into Functions
  1. Results indicate that the main function of gapcp1 is to supply 3-PGA for anabolic pathways in plastids of heterotrophic cells and suggest that the plastidial glycolysis may contribute to fatty acid biosynthesis in seeds. PMID: 27984670
  2. NO reduces meristem size but increases cell size predominantly in cortex layer in roots, suggesting some degree of cell specificity. Additionally, it is shown that NO modifies platidial GAPDH to regulate primary root growth.. [plastidial glyceraldehyde-3-phosphate dehydrogenase] PMID: 28478036
  3. Results indicate that GAPCp1 is not functionally important in photosynthetic cells but plays a fundamental role in roots and in heterotrophic cells of the aerial part of Arabidopsis. PMID: 26134167
  4. isoform GapC1 is a phosphorylating NAD-specific GAPDH with enzymatic activity strictly dependent on Cys(149) PMID: 22607208
Subcellular Location
Plastid, chloroplast stroma.
Protein Families
Glyceraldehyde-3-phosphate dehydrogenase family
Tissue Specificity
Expressed in shoot and root vasculature, leaf veins and vascular tissue of flowers and siliques.
Database Links

KEGG: ath:AT1G79530

STRING: 3702.AT1G79530.1

UniGene: At.17116

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