gadC Antibody

Code CSB-PA351829XA01ENV
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Product Details

Full Product Name
Rabbit anti-Escherichia coli (strain K12) gadC Polyclonal antibody
Uniprot No.
Target Names
gadC
Alternative Names
gadC antibody; acsA antibody; xasA antibody; b1492 antibody; JW1487 antibody; Probable glutamate/gamma-aminobutyrate antiporter antibody; Extreme acid sensitivity protein antibody
Raised in
Rabbit
Species Reactivity
Escherichia coli (strain K12)
Immunogen
Recombinant Escherichia coli (strain K12) gadC protein
Immunogen Species
Escherichia coli (strain K12)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Involved in glutaminase-dependent acid resistance. Exchanges extracellular glutamate (Glu) for intracellular gamma-aminobutyric acid (GABA) under acidic conditions. The protonation states of substrates are crucial for transport. Selectively transports Glu with no net charge and GABA with a positive charge. Also efficiently transports glutamine and, to a smaller extent, methionine and leucine. When the extracellular pH drops below 2.5, can import L-glutamine and export either glutamate or GABA. The ability to survive the extremely acidic conditions of the stomach is essential for successful colonization of the host by commensal and pathogenic bacteria.
Gene References into Functions
  1. GadC selectively exchanges Glu(-) or Glu(0) with GABA(+), resulting in effective proton extrusion of >0.9 H(+) per turnover to counter proton invasion into acid-challenged bacteria PMID: 23530225
Subcellular Location
Cell inner membrane; Multi-pass membrane protein.
Protein Families
Amino acid-polyamine-organocation (APC) superfamily, Glutamate:GABA antiporter (GGA) (TC 2.A.3.7) family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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