gag Antibody

Datasheet

Code	CSB-PA529602XA01ROQ
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Product Details

Full Product Name

Rabbit anti-Rous sarcoma virus (strain Schmidt-Ruppin B) (RSV-SRB) gag Polyclonal antibody

Uniprot No.

O92954

Target Names

gag

Alternative Names

gag antibody; Gag polyprotein [Cleaved into: Matrix protein p19; p2A; p2B; p10; Capsid protein p27 antibody; alternate cleaved 1; Capsid protein p27 antibody; alternate cleaved 2; Spacer peptide antibody; SP antibody; p3); Nucleocapsid protein p12; Protease p15 antibody; EC 3.4.23.-)] antibody

Raised in

Rabbit

Species Reactivity

Rous sarcoma virus (strain Schmidt-Ruppin B) (RSV-SRB)

Immunogen

Recombinant Rous sarcoma virus (strain Schmidt-Ruppin B) (RSV-SRB) gag protein

Immunogen Species

Rous sarcoma virus (strain Schmidt-Ruppin B) (RSV-SRB)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

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Target Background

Function

The p10 domain folds back and interacts with the capsid protein domain during Gag polyprotein assembly in the immature particle (before the maturation cleavage that splits the 2 domains).; Capsid protein p27: Self-associates to form the irregular polyhedron core composed of hexamers and pentamers, that encapsulates the genomic RNA-nucleocapsid complex. Assembles as a tube in vitro.; Binds strongly to viral nucleic acids and promote their aggregation. Also destabilizes the nucleic acids duplexes via highly structured zinc-binding motifs.; Plays a role in the oligomerization of the Gag polyprotein and in the stabilization of the immature particle. Essential layering element during tube assembly.; Aspartyl protease that mediates proteolytic cleavages of Gag and Gag-Pol polyproteins during or shortly after the release of the virion from the plasma membrane. Cleavages take place as an ordered, step-wise cascade to yield mature proteins. This process is called maturation. Displays maximal activity during the budding process just prior to particle release from the cell.

Subcellular Location

[Matrix protein p19]: Virion.; [Capsid protein p27, alternate cleaved 1]: Virion.; [Capsid protein p27, alternate cleaved 2]: Virion.; [Nucleocapsid protein p12]: Virion.; [Gag polyprotein]: Host nucleus, host nucleolus. Host nucleus, host nucleoplasm.

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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gag Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background