gag Antibody

Code CSB-PA304026XA01EFT
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Product Details

Full Product Name
Rabbit anti-Equine infectious anemia virus (isolate CL22) (EIAV) gag Polyclonal antibody
Uniprot No.
Target Names
gag
Alternative Names
gag antibody; Gag polyprotein [Cleaved into: Matrix protein p15 antibody; MA); Capsid protein p26 antibody; CA); p1; Nucleocapsid protein p11 antibody; NC); p9] antibody
Raised in
Rabbit
Species Reactivity
Equine infectious anemia virus (isolate CL22) (EIAV)
Immunogen
Recombinant Equine infectious anemia virus (isolate CL22) (EIAV) gag protein
Immunogen Species
Equine infectious anemia virus (isolate CL22) (EIAV)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Matrix protein p15 forms the outer shell of the core of the virus, lining the inner surface of the viral membrane.; Capsid protein p26 forms the conical core of the virus that encapsulates the genomic RNA-nucleocapsid complex.; Nucleocapsid protein p11 encapsulates and protects viral dimeric unspliced (genomic) RNA. Binds these RNAs through its zinc fingers.; p9 plays a role in budding of the assembled particle by interacting with PDCD6IP/AIP1.
Gene References into Functions
  1. Equine infectious anemia virus Gag is sumoylated at lysine 30 of its p9 domain. PMID: 28342872
  2. Then, the authors determined that W7 and K9 in the equine infectious anemia virus matrix protein N terminus were essential for Gag assembly and release but did not affect the cellular distribution of Gag. PMID: 26637458
  3. EIAV p9 Gag protein mediates virus release by recruiting the ALG2-interacting protein X (ALIX) via the YPDL-motif to the site of virus budding, the counterpart of the YPXnL-motif found in p6. PMID: 20015412
  4. lentivirus assembly and budding are regulated by the RNA nuclear export pathway and alternative cellular pathways can be adapted for lentiviral Gag assembly and budding. PMID: 17686839
  5. A phylogenetic analysis based on 414nts of Gag gene sequences amplified by a nested PCR revealed the distinct divergence of these variants compared to other published strains in a corresponding region PMID: 17767972

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Subcellular Location
Virion.
Protein Families
Equine lentivirus group gag polyprotein family
Database Links

KEGG: vg:1489984

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