galK Antibody

Code CSB-PA009198XA01ENV
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Product Details

Full Product Name
Rabbit anti-Escherichia coli (strain K12) galK Polyclonal antibody
Uniprot No.
Target Names
galK
Alternative Names
galK antibody; galA antibody; b0757 antibody; JW0740 antibody; Galactokinase antibody; EC 2.7.1.6 antibody; Galactose kinase antibody
Raised in
Rabbit
Species Reactivity
Escherichia coli (strain K12)
Immunogen
Recombinant Escherichia coli (strain K12) galK protein
Immunogen Species
Escherichia coli (strain K12)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Catalyzes the transfer of the gamma-phosphate of ATP to D-galactose to form alpha-D-galactose-1-phosphate (Gal-1-P). To a lesser extent, is also able to phosphorylate 2-deoxy-D-galactose and D-galactosamine. Is not able to use D-galacturonic acid, D-talose, L-altrose, and L-glucose as substrates.
Gene References into Functions
  1. type 2 polarity occurs as a result of the down-regulation of galK, which is caused by two mechanisms: Spot 42-mediated degradation of the galK-specific mRNA, mK2, and Spot 42-mediated Rho-dependent transcription termination at the end of galT PMID: 26045496
  2. 6-azido-6-deoxy-galactose and 6-azido-6-deoxy-glucose present unique chemical probes to assess the utility of an E. coli Y371H/M173L-GalK-overproducing strain to generate unnatural sugar-1-phosphates in vivo PMID: 15975511
Subcellular Location
Cytoplasm.
Protein Families
GHMP kinase family, GalK subfamily
Database Links

KEGG: ecj:JW0740

STRING: 316407.1651342

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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