GAL80 Antibody

Code CSB-PA356247XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) GAL80 Polyclonal antibody
Uniprot No.
Target Names
GAL80
Alternative Names
GAL80 antibody; YML051W antibody; YM9827.01 antibody; YM9958.12Galactose/lactose metabolism regulatory protein GAL80 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) GAL80 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
This protein is a negative regulator for the gene expression of the lactose/galactose metabolic genes. It binds to GAL4 and so blocks transcriptional activation by it, in the absence of an inducing sugar.
Gene References into Functions
  1. Here, the authors demonstrate that altering the epistatic interaction between Gal80p and Gal4p also abolishes the bistability of the GAL/MEL genetic switch, and the switch response to galactose becomes graded instead of binary. PMID: 27573383
  2. GAL switch becomes independent of GAL3 by altering the interaction between Gal4p and Gal80p. PMID: 25135592
  3. to understand mechanism of GAL regulatory system, structure of Gal80p with activation domain of Gal4p was determined; structure reveals existence of a dinucleotide mediating interaction between the two; findings suggest NADP plays a key role PMID: 18292341
  4. Both MgATP and galactose are required to promote the formation of a 1:1 complex between Gal80p and Gal3p. PMID: 11964151
Database Links

KEGG: sce:YML051W

STRING: 4932.YML051W

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