Gale Antibody

Code CSB-PA894763XA01DLU
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Product Details

Full Product Name
Rabbit anti-Drosophila melanogaster (Fruit fly) Gale Polyclonal antibody
Uniprot No.
Target Names
Gale
Alternative Names
Gale antibody; CG12030UDP-glucose 4-epimerase antibody; EC 5.1.3.2 antibody; Galactowaldenase antibody; UDP-N-acetylgalactosamine 4-epimerase antibody; UDP-GalNAc 4-epimerase antibody; UDP-N-acetylglucosamine 4-epimerase antibody; UDP-GlcNAc 4-epimerase antibody; EC 5.1.3.7 antibody; UDP-galactose 4-epimerase antibody
Raised in
Rabbit
Species Reactivity
Drosophila melanogaster (Fruit fly)
Immunogen
Recombinant Drosophila melanogaster (Fruit fly) Gale protein
Immunogen Species
Drosophila melanogaster (Fruit fly)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Catalyzes two distinct but analogous reactions: the reversible epimerization of UDP-glucose to UDP-galactose and the reversible epimerization of UDP-N-acetylglucosamine to UDP-N-acetylgalactosamine. The reaction with UDP-Gal plays a critical role in the Leloir pathway of galactose catabolism in which galactose is converted to the glycolytic intermediate glucose 6-phosphate. It contributes to the catabolism of dietary galactose and enables the endogenous biosynthesis of both UDP-Gal and UDP-GalNAc when exogenous sources are limited. Both UDP-sugar interconversions are important in the synthesis of glycoproteins and glycolipids.
Gene References into Functions
  1. Our results demonstrate that both UDP-gal and UDP-galNAc activities of dGALE are required for Drosophila survival, although distinct roles for each activity can be seen in specific windows of developmental time or in response to a galactose challenge. PMID: 22654673
  2. Drosophila with partial GALE loss survive in the absence of galactose but succumb in development if exposed to dietary galactose. PMID: 20519568
Protein Families
NAD(P)-dependent epimerase/dehydratase family
Database Links

KEGG: dme:Dmel_CG12030

STRING: 7227.FBpp0072455

UniGene: Dm.4155

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