GA20OX1 Antibody

Code CSB-PA657879XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) GA20OX1 Polyclonal antibody
Uniprot No.
Target Names
GA20OX1
Alternative Names
GA20OX1 antibody; 20ox1 antibody; At2301 antibody; GA5 antibody; At4g25420 antibody; T30C3.90 antibody; Gibberellin 20 oxidase 1 antibody; EC 1.14.11.- antibody; GA 20-oxidase 1 antibody; AtGA20ox1 antibody; Gibberellin C-20 oxidase 1 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana GA20OX1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Key oxidase enzyme in the biosynthesis of gibberellin that catalyzes the conversion of GA12 and GA53 to GA9 and GA20 respectively, via a three-step oxidation at C-20 of the GA skeleton. GA53 is less effectively oxidized than GA12, and GA25 is also formed as a minor product. Involved in the promotion of the floral transition, fertility and silique elongation, but plays only a minor role in elongation of seedling organs. Acts redundantly with GA20OX2.
Gene References into Functions
  1. GA20ox1 expression affects gibberellin responses. PMID: 27879393
  2. We propose that cytokinin activity increased due to a KNOX-mediated transcriptional feedback loop resulting from the higher GNP1 transcript levels, in turn leading to increased expression of the GA catabolism genes GA2oxs and reduced GA1 and GA3 accumulation. PMID: 27764111
  3. GA20-OX1 plants accumulated more vegetative biomass than control plants in greenhouse experiments. PMID: 26903034
  4. Mutations in the early steps of the GA biosynthesis pathway led to a reduction in shoot as well as root size. Mutations at the GA5 locus yielded phenotypes characterized by decreased root length in comparison with related wild-type ones. PMID: 26162399
  5. A loss-of-function mutation due to a single nucleotide deletion in gibberellin20-oxidase1 (GA5) was identified as the cause of dwarfism in an alpine accession. PMID: 25941313
  6. antagonistic pleiotropy might underlie the occurrence of GA5 loss-of-function mutations in nature. PMID: 24023067
  7. Loss of GA20ox1, -2, and -3 blocks germination, causes severe dwarfism, delays flowering, and causes male and female sterility. PMID: 22427334
  8. Studies indicate that GA5 gene encodes a GA 20-oxidase that catalyzes the formation of the GA20 and GA9. PMID: 21103336
  9. Data show AtGA20ox1 and AtGA20ox2 act redundantly to promote hypocotyl and internode elongation, flowering time, elongation of anther filaments, the number of seeds that develop per silique and elongation of siliques. PMID: 18069939
  10. Expression of GA20ox1 is regulated at multiple levels. PMID: 18440929

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Protein Families
Iron/ascorbate-dependent oxidoreductase family, GA20OX subfamily
Tissue Specificity
Highly expressed in stems and inflorescence tissues. Detected in seeds, roots, leaves and siliques.
Database Links

KEGG: ath:AT4G25420

STRING: 3702.AT4G25420.1

UniGene: At.32258

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