GAT1 Antibody

Code CSB-PA331873XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) GAT1 Polyclonal antibody
Uniprot No.
Target Names
GAT1
Alternative Names
GAT1 antibody; YFL021W antibody; Transcriptional regulatory protein GAT1 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) GAT1 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
Positive regulator of multiple nitrogen catabolic genes.
Gene References into Functions
  1. Based on these results, the genetic engineering on Gat1p has a great potential in enhancing non-preferred nitrogen metabolism in S. cerevisiae. PMID: 26899143
  2. Data show that the GAT1 gene, encoding a transcriptional activator of nitrogen-responsive catabolic genes, produces a variety of mRNAs differing in their 5' and 3' termini. PMID: 26259534
  3. We demonstrate that wild type Gat1 isoforms (IsoA and IsoB) are initiated at Gat1 methionines 40, 95, and/or 102, but not at methionine 1 regardless of nitrogen conditions. PMID: 24324255
  4. Rapamycin-dependent Gat1 and Gln3 binding to the Aro80 target promoters is not affected by tryptophan availability, suggesting that transactivation activity of Aro80 is not necessary for the recruitment of GATA factors. PMID: 23651256
  5. Gln3, Gat1, and Ure2 respond to TorC1 and nitrogen availability via distinct regulatory pathways. PMID: 23184930
  6. Controlling lipid fluxes at glycerol-3-phosphate acyltransferase step in yeast: unique contribution of Gat1p to oleic acid-induced lipid particle formation. PMID: 22267742
  7. Nitrogen-responsive regulation of GATA protein family activators Gln3 and Gat1 occurs by two distinct pathways, one inhibited by rapamycin and the other by methionine sulfoximine. PMID: 22039046
  8. Gln3 and Gat1 localizations are controlled by two different regulatory pathways PMID: 20378536
  9. role in regulation of invertase activity and SUC2 expression PMID: 15780659
  10. Rtg1/3p, Gln3p, and Gat1p can be differentially regulated through different nutrient-sensing pathways, such as TOR and retrograde signaling, and by multiple factors, such as Lst8p, which is suggested to have a role in connecting the RTG and TOR pathways PMID: 16253991
  11. in the absence of urmylation, nuclear/cytosolic shuffling of both Nil1 and Gln3 transcriptional factors is altered, ultimately leading to inability to repress GAP1 gene PMID: 17254574
  12. Tor pathway control of the nitrogen-responsive DAL5 gene bifurcates at the level of Gln3 and Gat1 regulation in Saccharomyces cerevisiae PMID: 18245087
  13. Results show that Gat1 is a key factor for the integrated control of nitrogen catabolite repression in Saccharomyces cerevisiae. PMID: 19380492
  14. The phosphorylation of Put3p appears to influence the association of Gat1p, but not Gln3p, to the PUT1 promoter. PMID: 19574222

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Subcellular Location
Nucleus.
Database Links

KEGG: sce:YFL021W

STRING: 4932.YFL021W

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